Production and applications of antibodies directed against the chiral center of α-amino acids

O. Hofstetter*, H. Hofstetter, M. Wilchek, V. Schurig, B. S. Green

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

In this paper we demonstrate the suitability of antibodies as tailor-made chiral selectors. Polyclonal and monoclonal antibodies which stereoselectively bind to the functional grouping of α-amino acids were raised and used for the detection and chiral separation of amino acid enantiomers. The antibodies were used in various analytical applications, including immunoassay and sensor approaches, as well as chromatography. Due to their excellent stereoselectivity the antibodies allow the detection of enantiomer ratios at a level that exceeds currently available methods.

Original languageEnglish
Pages (from-to)165-174
Number of pages10
JournalInternational Journal of Bio-Chromatography
Volume5
Issue number3
StatePublished - 2000
Externally publishedYes

Keywords

  • Amino acid
  • Antibody
  • Chiral discrimination
  • Chromatography
  • Immunoassay
  • Sensor

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