Programmed cell death in Escherichia coli: Some antibiotics can trigger mazEF lethality

B. Sat, R. Hazan, T. Fisher, H. Khaner, G. Glaser, H. Engelberg-Kulka*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

200 Scopus citations


The discovery of toxin-antitoxin gene pairs (also called addiction modules) on extrachromosomal elements of Escherichia coli, and particularly the discovery of homologous modules on the bacterial chromosome, suggest that a potential for programmed cell death may be inherent in bacterial cultures. We have reported on the E. coli mazEF system, a regulatable addiction module located on the bacterial chromosome. MazF is a stable toxin and MazE is a labile antitoxin. Here we show that cell death mediated by the E. coli mazEF module can be triggered by several antibiotics (rifampicin, chloramphenicol, and spectinomycin) that are general inhibitors of transcription and/or translation. These antibiotics inhibit the continuous expression of the labile antitoxin MazE, and as a result, the stable toxin MazF causes cell death. Our results have implications for the possible mode(s) of action of this group of antibiotics.

Original languageAmerican English
Pages (from-to)2041-2045
Number of pages5
JournalJournal of Bacteriology
Issue number6
StatePublished - 2001


Dive into the research topics of 'Programmed cell death in Escherichia coli: Some antibiotics can trigger mazEF lethality'. Together they form a unique fingerprint.

Cite this