Programming cell growth into different cluster shapes using diffusible signals

Yipei Guo*, Mor Nitzan, Michael P. Brenner

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Advances in genetic engineering technologies have allowed the construction of artificial genetic circuits, which have been used to generate spatial patterns of differential gene expression. However, the question of how cells can be programmed, and how complex the rules need to be, to achieve a desired tissue morphology has received less attention. Here, we address these questions by developing a mathematical model to study how cells can collectively grow into clusters with different structural morphologies by secreting diffusible signals that can influence cellular growth rates. We formulate how growth regulators can be used to control the formation of cellular protrusions and how the range of achievable structures scales with the number of distinct signals. We show that a single growth inhibitor is insufficient for the formation of multiple protrusions but may be achieved with multiple growth inhibitors, and that other types of signals can regulate the shape of protrusion tips. These examples illustrate how our approach could potentially be used to guide the design of regulatory circuits for achieving a desired target structure.

Original languageAmerican English
Article numbere1009576
JournalPLoS Computational Biology
Issue number11
StatePublished - Nov 2021
Externally publishedYes

Bibliographical note

Publisher Copyright:
Copyright © 2021 Guo et al.


Dive into the research topics of 'Programming cell growth into different cluster shapes using diffusible signals'. Together they form a unique fingerprint.

Cite this