Progression of meiosis is coordinated by the level and location of MAPK activation via OGR-2 in Caenorhabditis elegans

Hanna Achache, Lévana Laurent, Yaël Hecker-Mimoun, Hasan Ishtayeh, Yisrael Rappaport, Eitan Kroizer, Monica P. Colaiácovo, Yonatan B. Tzur*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

During meiosis, a series of evolutionarily conserved events allow for reductional chromosome division, which is required for sexual reproduction. Although individual meiotic processes have been extensively studied, we currently know far less about how meiosis is regulated and coordinated. In the Caenorhabditis elegans gonad, mitogen-activated protein kinase (MAPK) signaling drives oogenesis while undergoing spatial activation and deactivation waves. However, it is currently unclear how MAPK activation is governed and how it facilitates the progression of oogenesis. Here, we show that the oocyte and germline-related 2 (ogr-2) gene affects proper progression of oogenesis. Complete deletion of ogr-2 results in delayed meiotic entry and late spatial onset of double-strand break repair. Elevated levels of apoptosis are observed in this mutant, independent of the meiotic canonical checkpoints; however, they are dependent on the MAPK terminal member MPK-1/ERK. MPK-1 activation is elevated in diplotene in ogr-2 mutants and its aberrant spatial activation correlates with stages where meiotic progression defects are evident. Deletion of ogr-2 significantly reduces the expression of lip-1, a phosphatase reported to repress MPK-1, which is consistent with OGR-2 localization at chromatin in germ cells. We suggest that OGR-2 modulates the expression of lip-1 to promote the timely progression of meiosis through MPK-1 spatial deactivation.

Original languageAmerican English
Pages (from-to)213-229
Number of pages17
JournalGenetics
Volume212
Issue number1
DOIs
StatePublished - May 2019

Bibliographical note

Publisher Copyright:
© 2019, Genetics Society of America. All rights reserved.

Keywords

  • Fertility
  • Meiosis
  • Oogenesis

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