Prolonged feeding with green tea polyphenols exacerbates cholesterol-induced fatty liver disease in mice

Nina Hirsch, Anya Konstantinov, Sarit Anavi, Anna Aronis, Zion Hagay, Zecharia Madar, Oren Tirosh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

36 Scopus citations


Scope: This study investigated the potential deleterious impact of dietary supplementation with green tea extract (GTE) on the progression of fatty liver disease, in a mouse model of cholesterol-induced steatohepatitis that represents chronic liver injury. Methods and results: Male C57BL mice (n = 32, 8-wk-old) were fed for 6 wk with one of the following diets: normal control diet (ND, Con), Con + 1% w/w polyphenols from GTE (Con + GTE); high cholesterol diet, Con + 1% cholesterol + 0.5% cholate w/w (HCD); HCD + 1% green tea polyphenols w/w (HCD + GTE). Hepatic steatosis, oxidative, and inflammatory markers and bile acid synthesis pathways were measured. HCD supplementation resulted in hepatic steatosis and liver damage. In animals supplemented with the HCD + GTE an exacerbated hepatic steatosis, oxidative stress, and inflammatory response were observed compared to HCD supplemented animals. HCD + GTE supplementation elevated blood levels of liver enzymes and serum bile acids compared HCD-treated animals. HCD + GTE supplementation altered bile acid synthesis in the cholesterol clearance pathway, inducing a shift from the classically regulated CYP7A1 pathway to the alternative acidic pathway. Conclusion: Prolonged GTE supplementation dramatically increased hepatic oxidative stress, inflammation and liver injury, and altered the bile acid synthesis pathway in mice fed a HCD.

Original languageAmerican English
Pages (from-to)2542-2553
Number of pages12
JournalMolecular Nutrition and Food Research
Issue number12
StatePublished - 1 Dec 2016

Bibliographical note

Publisher Copyright:
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim


  • Lipotoxicity
  • Metabolism
  • Nonalcoholic steatohepatitis
  • Nutrition
  • Polyphenols


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