Prolonged transgene expression in murine salivary glands following non-primate lentiviral vector transduction

Ela Shai, Aaron Palmon, Amos Panet, Yitzhak Marmary, Yoav Sherman, Michael A. Curran, Eithan Galun, Reba Condiotti*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


Salivary glands are an accessible organ for gene therapy, enabling expression of recombinant proteins for both exocrine and endocrine secretion. Lentivirus-based vectors have many advantages for gene therapy, including their ability to infect nondividing cells and to stably integrate into the host genome, enabling long-term transgene expression without eliciting an inflammatory immune response. In the present study, murine salivary glands were inoculated with feline immunodeficiency virus (FIV)-based lentiviral vectors expressing various reporter genes. Luciferase expression was observed as early as 24 h posttransduction, peaked at 17-21 days, and remained stable for more than 80 days. Staining with X-gal suggested that mucous acinar cells were effectively transduced. FIV vector transduction with the secreted alkaline phosphatase gene increased serum levels in treated animals for up to 45 days, and the FIV vector harboring the interferon-γ (IFN-γ) expression cassette induced an increase in IFN-γ serum levels as well as in the supernatant of salivary gland explant cultures. These results demonstrate that the transduction of salivary glands with nonprimate lentiviral vectors may provide a novel and highly effective vehicle for long-term endocrine transgene expression.

Original languageAmerican English
Pages (from-to)137-143
Number of pages7
JournalMolecular Therapy
Issue number1
StatePublished - Jul 2005


  • Exocrine and endocrine secretion
  • Feline immunodeficiency virus
  • Mucous and serous cell transduction
  • Submandibular and sublingual glands
  • Viral integration


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