TY - JOUR
T1 - Promoters of Mycoplasma capricolum ribosomal RNA operons
T2 - Identical activities but different regulation in homologous and heterologous cells
AU - Gafny, Ron
AU - Hyman, Hana C.
AU - Razin, Shmuel
AU - Glaser, Gad
PY - 1988/1/11
Y1 - 1988/1/11
N2 - The 5' region of the rRNA operon, rrnA, of M. capricolum was cloned. Sequence analysis revealed two tRNA genes, tRNAleu and tRNAlys, upstream to the promoter of the rRNA operon. The in vivo transcription start sites of the rRNA operon and of the tRNA genes were mapped. The same promoters used by M. capricolum RNA polymerase are also recognized by E. coli RNA polymerase both in vivo and in vitro. We find that high levels of ppGpp in E. coll, resulting from amino acid starvation or from spoT mutation, activate rather than repress the transcription of the mycoplasma rrnA operon.
AB - The 5' region of the rRNA operon, rrnA, of M. capricolum was cloned. Sequence analysis revealed two tRNA genes, tRNAleu and tRNAlys, upstream to the promoter of the rRNA operon. The in vivo transcription start sites of the rRNA operon and of the tRNA genes were mapped. The same promoters used by M. capricolum RNA polymerase are also recognized by E. coli RNA polymerase both in vivo and in vitro. We find that high levels of ppGpp in E. coll, resulting from amino acid starvation or from spoT mutation, activate rather than repress the transcription of the mycoplasma rrnA operon.
UR - http://www.scopus.com/inward/record.url?scp=0024283920&partnerID=8YFLogxK
U2 - 10.1093/nar/16.1.61
DO - 10.1093/nar/16.1.61
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C2 - 3340543
AN - SCOPUS:0024283920
SN - 0305-1048
VL - 16
SP - 61
EP - 76
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 1
ER -