Abstract
Kinetic parameters of the chloroplastbound ferredoxin-NADP+ reductase from two varieties of wheat (Triticum aestivum), hardy Kharkov 22 MC (winter wheat) and less hardy Rescue (spring wheat), were followed during induction of frost hardiness as a means of examining possible changes in chloroplast membranes during hardening. No changes were found in the Michaelis constants for NADPH and 2,6-dichlorophenol indophenol, inhibition constants for p-chloromercuriphenylsulfonate, and activation energy values of the enzyme in either variety. The data suggest that no qualitative changes occurred in the properties of wheat chloroplast membranes related to ferredoxin-NADP+ reductase during cold hardening.
Original language | English |
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Pages (from-to) | 80-86 |
Number of pages | 7 |
Journal | Cryobiology |
Volume | 15 |
Issue number | 1 |
DOIs | |
State | Published - Feb 1978 |
Externally published | Yes |