Prostaglandin E1-stimulable cyclic AMP formation from rat gastric antral organ culture: Lack of effect on gastrin secretion

S. A. Lamprecht; J. Krawiec; B. Schwartz; P. Krugliak; J. Goldstein; H. S. Odes

doi:10.1016/0006-291X(82)91849-6

Prostaglandin E₁-stimulable cyclic AMP formation from rat gastric antral organ culture: Lack of effect on gastrin secretion

S. A. Lamprecht^*, J. Krawiec, B. Schwartz, P. Krugliak, J. Goldstein, H. S. Odes

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Explants from rat antral mucosae in organ culture were exposed to prostaglandin E₁ (2.8 × 10^-5M) for 12 h. Prostaglandin E₁ significantly stimulated the formation of cyclic AMP (pmol/mg tissue ± S.E.M.): 17.4 ± 2.5 in prostaglandin-treated samples compared to 5.5 ± 0.3 in control specimens. The increase in tissue cyclic AMP was not associated with enhanced release of immunoreactive gastrin into the culture media. Addition of isoproterenol (1 × 10^-5M) to the bathing media resulted in a 4-fold increase in antral cyclic AMP production. In contrast to prostaglandin E₁, isoproterenol exerted a marked stimulatory action on gastrin secretion during a 12 h culture period. Mucosal explants cultured for variable periods of time (6-12 h) in the presence of 8-Br-cyclic AMP (5mM) responded with a 2-fold rise in gastrin release. The results suggest that prostaglandin E₁ enhances the level of cyclic AMP in sequestered cellular compartments not functionally linked to gastrin secretory mechanisms.

Original language	English
Pages (from-to)	186-192
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	108
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(82)91849-6
State	Published - 16 Sep 1982
Externally published	Yes

Bibliographical note

Funding Information:
We are grateful to Mrs. R. Berladski for skillful technical assistance and Mrs. B. Save11 for expert editorial assistance. The work was supported in part by a grant of the Bat-Sheva de Rothschild Fund for the Advancement of Science and Technology, Jerusalem.

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10.1016/0006-291X(82)91849-6

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title = "Prostaglandin E1-stimulable cyclic AMP formation from rat gastric antral organ culture: Lack of effect on gastrin secretion",

abstract = "Explants from rat antral mucosae in organ culture were exposed to prostaglandin E1 (2.8 × 10-5M) for 12 h. Prostaglandin E1 significantly stimulated the formation of cyclic AMP (pmol/mg tissue ± S.E.M.): 17.4 ± 2.5 in prostaglandin-treated samples compared to 5.5 ± 0.3 in control specimens. The increase in tissue cyclic AMP was not associated with enhanced release of immunoreactive gastrin into the culture media. Addition of isoproterenol (1 × 10-5M) to the bathing media resulted in a 4-fold increase in antral cyclic AMP production. In contrast to prostaglandin E1, isoproterenol exerted a marked stimulatory action on gastrin secretion during a 12 h culture period. Mucosal explants cultured for variable periods of time (6-12 h) in the presence of 8-Br-cyclic AMP (5mM) responded with a 2-fold rise in gastrin release. The results suggest that prostaglandin E1 enhances the level of cyclic AMP in sequestered cellular compartments not functionally linked to gastrin secretory mechanisms.",

author = "Lamprecht, {S. A.} and J. Krawiec and B. Schwartz and P. Krugliak and J. Goldstein and Odes, {H. S.}",

note = "Funding Information: We are grateful to Mrs. R. Berladski for skillful technical assistance and Mrs. B. Save11 for expert editorial assistance. The work was supported in part by a grant of the Bat-Sheva de Rothschild Fund for the Advancement of Science and Technology, Jerusalem.",

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T2 - Lack of effect on gastrin secretion

AU - Lamprecht, S. A.

AU - Krawiec, J.

AU - Schwartz, B.

AU - Krugliak, P.

AU - Goldstein, J.

AU - Odes, H. S.

N1 - Funding Information: We are grateful to Mrs. R. Berladski for skillful technical assistance and Mrs. B. Save11 for expert editorial assistance. The work was supported in part by a grant of the Bat-Sheva de Rothschild Fund for the Advancement of Science and Technology, Jerusalem.

PY - 1982/9/16

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N2 - Explants from rat antral mucosae in organ culture were exposed to prostaglandin E1 (2.8 × 10-5M) for 12 h. Prostaglandin E1 significantly stimulated the formation of cyclic AMP (pmol/mg tissue ± S.E.M.): 17.4 ± 2.5 in prostaglandin-treated samples compared to 5.5 ± 0.3 in control specimens. The increase in tissue cyclic AMP was not associated with enhanced release of immunoreactive gastrin into the culture media. Addition of isoproterenol (1 × 10-5M) to the bathing media resulted in a 4-fold increase in antral cyclic AMP production. In contrast to prostaglandin E1, isoproterenol exerted a marked stimulatory action on gastrin secretion during a 12 h culture period. Mucosal explants cultured for variable periods of time (6-12 h) in the presence of 8-Br-cyclic AMP (5mM) responded with a 2-fold rise in gastrin release. The results suggest that prostaglandin E1 enhances the level of cyclic AMP in sequestered cellular compartments not functionally linked to gastrin secretory mechanisms.

AB - Explants from rat antral mucosae in organ culture were exposed to prostaglandin E1 (2.8 × 10-5M) for 12 h. Prostaglandin E1 significantly stimulated the formation of cyclic AMP (pmol/mg tissue ± S.E.M.): 17.4 ± 2.5 in prostaglandin-treated samples compared to 5.5 ± 0.3 in control specimens. The increase in tissue cyclic AMP was not associated with enhanced release of immunoreactive gastrin into the culture media. Addition of isoproterenol (1 × 10-5M) to the bathing media resulted in a 4-fold increase in antral cyclic AMP production. In contrast to prostaglandin E1, isoproterenol exerted a marked stimulatory action on gastrin secretion during a 12 h culture period. Mucosal explants cultured for variable periods of time (6-12 h) in the presence of 8-Br-cyclic AMP (5mM) responded with a 2-fold rise in gastrin release. The results suggest that prostaglandin E1 enhances the level of cyclic AMP in sequestered cellular compartments not functionally linked to gastrin secretory mechanisms.

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Prostaglandin E₁-stimulable cyclic AMP formation from rat gastric antral organ culture: Lack of effect on gastrin secretion

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