Explants from rat antral mucosae in organ culture were exposed to prostaglandin E1 (2.8 × 10-5M) for 12 h. Prostaglandin E1 significantly stimulated the formation of cyclic AMP (pmol/mg tissue ± S.E.M.): 17.4 ± 2.5 in prostaglandin-treated samples compared to 5.5 ± 0.3 in control specimens. The increase in tissue cyclic AMP was not associated with enhanced release of immunoreactive gastrin into the culture media. Addition of isoproterenol (1 × 10-5M) to the bathing media resulted in a 4-fold increase in antral cyclic AMP production. In contrast to prostaglandin E1, isoproterenol exerted a marked stimulatory action on gastrin secretion during a 12 h culture period. Mucosal explants cultured for variable periods of time (6-12 h) in the presence of 8-Br-cyclic AMP (5mM) responded with a 2-fold rise in gastrin release. The results suggest that prostaglandin E1 enhances the level of cyclic AMP in sequestered cellular compartments not functionally linked to gastrin secretory mechanisms.
|Original language||American English|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - 16 Sep 1982|
Bibliographical noteFunding Information:
We are grateful to Mrs. R. Berladski for skillful technical assistance and Mrs. B. Save11 for expert editorial assistance. The work was supported in part by a grant of the Bat-Sheva de Rothschild Fund for the Advancement of Science and Technology, Jerusalem.