Proteasome lid bridges mitochondrial stress with Cdc53/Cullin1 NEDDylation status

L. Bramasole, A. Sinha, S. Gurevich, M. Radzinski, Y. Klein, N. Panat, E. Gefen, T. Rinaldi, D. Jimenez-Morales, J. Johnson, N. J. Krogan, N. Reis, D. Reichmann, M. H. Glickman, E. Pick*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


Cycles of Cdc53/Cullin1 rubylation (a.k.a NEDDylation) protect ubiquitin-E3 SCF (Skp1-Cullin1-F-box protein) complexes from self-destruction and play an important role in mediating the ubiquitination of key protein substrates involved in cell cycle progression, development, and survival. Cul1 rubylation is balanced by the COP9 signalosome (CSN), a multi-subunit derubylase that shows 1:1 paralogy to the 26S proteasome lid. The turnover of SCF substrates and their relevance to various diseases is well studied, yet, the extent by which environmental perturbations influence Cul1 rubylation/derubylation cycles per se is still unclear. In this study, we show that the level of cellular oxidation serves as a molecular switch, determining Cullin1 rubylation/derubylation ratio. We describe a mutant of the proteasome lid subunit, Rpn11 that exhibits accumulated levels of Cullin1-Rub1 conjugates, a characteristic phenotype of csn mutants. By dissecting between distinct phenotypes of rpn11 mutants, proteasome and mitochondria dysfunction, we were able to recognize the high reactive oxygen species (ROS) production during the transition of cells into mitochondrial respiration, as a checkpoint of Cullin1 rubylation in a reversible manner. Thus, the study adds the rubylation cascade to the list of cellular pathways regulated by redox homeostasis.

Original languageAmerican English
Pages (from-to)533-543
Number of pages11
JournalRedox Biology
StatePublished - Jan 2019

Bibliographical note

Publisher Copyright:
© 2018 The Authors


  • 26S proteasome
  • Mitochondria
  • NEDD8/Rub1
  • Rpn11
  • Thiol switch
  • Ubiquitin


Dive into the research topics of 'Proteasome lid bridges mitochondrial stress with Cdc53/Cullin1 NEDDylation status'. Together they form a unique fingerprint.

Cite this