TY - JOUR
T1 - Protection of fibroblasts (NIH-3T3) against oxidative damage by cyanidin-3-rhamnoglucoside Isolated from fig fruits (Ficus carica L.)
AU - Solomon, Anat
AU - Golubowicz, Sara
AU - Yablowicz, Zeev
AU - Bergman, Margalit
AU - Grossman, Shlomo
AU - Altman, Arie
AU - Kerem, Zohar
AU - Flaishman, Moshe A.
PY - 2010/6/9
Y1 - 2010/6/9
N2 - Anthocyanins, plant secondary metabolites, have been recognized for their health-promoting properties when consumed by humans. In this study, the antioxidant properties of a major anthocyanin in fresh fig fruits, cyanidin-3-rhamnoglucoside (C3R), were evaluated by various assays in vitro and correlated with the protection afforded by C3R to cultured NIH-3T3 fibroblast cells. C3R inhibited lipid peroxidation from producing peroxy radicals (ROO •) and MDA in a dose-dependent manner, and a high calculated stoichiometric coefficient [n] for peroxy radicals was demonstrated. In addition to its scavenging of reactive oxygen species (ROS), C3R showed a strong chelating activity toward the Fe2+ ion. Finally, pretreatment with C3R inhibited proapoptotic processes that were initiated by the oxidation of lysosome membranes in fibroblast cells. The high antioxidant potential, with several modes of action of purified C3R, may contribute to health benefits gained by the consumption of fresh fig fruits.
AB - Anthocyanins, plant secondary metabolites, have been recognized for their health-promoting properties when consumed by humans. In this study, the antioxidant properties of a major anthocyanin in fresh fig fruits, cyanidin-3-rhamnoglucoside (C3R), were evaluated by various assays in vitro and correlated with the protection afforded by C3R to cultured NIH-3T3 fibroblast cells. C3R inhibited lipid peroxidation from producing peroxy radicals (ROO •) and MDA in a dose-dependent manner, and a high calculated stoichiometric coefficient [n] for peroxy radicals was demonstrated. In addition to its scavenging of reactive oxygen species (ROS), C3R showed a strong chelating activity toward the Fe2+ ion. Finally, pretreatment with C3R inhibited proapoptotic processes that were initiated by the oxidation of lysosome membranes in fibroblast cells. The high antioxidant potential, with several modes of action of purified C3R, may contribute to health benefits gained by the consumption of fresh fig fruits.
KW - Cyanidin-3-rhamnoglucoside (C3R)
KW - Fe chelation
KW - Fig fruits
KW - Linoleic acid oxidation
KW - Lysosome membrane stability
KW - NIH-3T3 fibroblasts
KW - ROS
UR - http://www.scopus.com/inward/record.url?scp=77953150487&partnerID=8YFLogxK
U2 - 10.1021/jf100122a
DO - 10.1021/jf100122a
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C2 - 20443626
AN - SCOPUS:77953150487
SN - 0021-8561
VL - 58
SP - 6660
EP - 6665
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 11
ER -