Proteins in Phloem Exudates from Photoperiodically Induced Pharbitis nil’ Seedlings

Haya Friedman; Eliezer E. Goldschmidt; Abraham H. Halevy

doi:10.1016/S0176-1617(87)80212-2

Proteins in Phloem Exudates from Photoperiodically Induced Pharbitis nil’ Seedlings

Haya Friedman, Eliezer E. Goldschmidt, Abraham H. Halevy^*

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Exudates were collected from 7-day-old Pharbitis nil seedlings. Using SDS-gel electrophoresis and silver stain technique, these exudates were found to contain six prominent proteins. The exudate proteins of photoperiodically induced plants (16 h darkness) were compared with exudate proteins of non-induced plants, either illuminated for 5 minutes at the middle of 16 h darkness, or grown under continuous illumination. No differences could be detected between the exudate proteins of induced and non-induced plants using both one and two dimensional gel electrophoresis.

Original language	English
Pages (from-to)	471-476
Number of pages	6
Journal	Journal of Plant Physiology
Volume	130
Issue number	4-5
DOIs	https://doi.org/10.1016/S0176-1617(87)80212-2
State	Published - 1987

Keywords

CL
continuous illumination
DDW
double distilled water
EDTA
Ethylenediaminetetra-acetic acid
exudate proteins
floral induction
Hydroxymethylaminomethane
I
induced
NB
night break
Pharbitis nil
Phenylmethyl sulfonyl fluoride
PMSF
Polyinylpolypyrrolidone
PVPP
SDS
SDS polyacryl-amid gel electrophoresis
SDS-PAGE
sodium dodecyl sulphate
Tris

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title = "Proteins in Phloem Exudates from Photoperiodically Induced Pharbitis nil{\textquoteright} Seedlings",

abstract = "Exudates were collected from 7-day-old Pharbitis nil seedlings. Using SDS-gel electrophoresis and silver stain technique, these exudates were found to contain six prominent proteins. The exudate proteins of photoperiodically induced plants (16 h darkness) were compared with exudate proteins of non-induced plants, either illuminated for 5 minutes at the middle of 16 h darkness, or grown under continuous illumination. No differences could be detected between the exudate proteins of induced and non-induced plants using both one and two dimensional gel electrophoresis.",

keywords = "CL, continuous illumination, DDW, double distilled water, EDTA, Ethylenediaminetetra-acetic acid, exudate proteins, floral induction, Hydroxymethylaminomethane, I, induced, NB, night break, Pharbitis nil, Phenylmethyl sulfonyl fluoride, PMSF, Polyinylpolypyrrolidone, PVPP, SDS, SDS polyacryl-amid gel electrophoresis, SDS-PAGE, sodium dodecyl sulphate, Tris",

author = "Haya Friedman and Goldschmidt, {Eliezer E.} and Halevy, {Abraham H.}",

year = "1987",

doi = "10.1016/S0176-1617(87)80212-2",

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AU - Friedman, Haya

AU - Goldschmidt, Eliezer E.

AU - Halevy, Abraham H.

PY - 1987

Y1 - 1987

N2 - Exudates were collected from 7-day-old Pharbitis nil seedlings. Using SDS-gel electrophoresis and silver stain technique, these exudates were found to contain six prominent proteins. The exudate proteins of photoperiodically induced plants (16 h darkness) were compared with exudate proteins of non-induced plants, either illuminated for 5 minutes at the middle of 16 h darkness, or grown under continuous illumination. No differences could be detected between the exudate proteins of induced and non-induced plants using both one and two dimensional gel electrophoresis.

AB - Exudates were collected from 7-day-old Pharbitis nil seedlings. Using SDS-gel electrophoresis and silver stain technique, these exudates were found to contain six prominent proteins. The exudate proteins of photoperiodically induced plants (16 h darkness) were compared with exudate proteins of non-induced plants, either illuminated for 5 minutes at the middle of 16 h darkness, or grown under continuous illumination. No differences could be detected between the exudate proteins of induced and non-induced plants using both one and two dimensional gel electrophoresis.

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KW - DDW

KW - double distilled water

KW - EDTA

KW - Ethylenediaminetetra-acetic acid

KW - exudate proteins

KW - floral induction

KW - Hydroxymethylaminomethane

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KW - NB

KW - night break

KW - Pharbitis nil

KW - Phenylmethyl sulfonyl fluoride

KW - PMSF

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KW - SDS polyacryl-amid gel electrophoresis

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ER -

Proteins in Phloem Exudates from Photoperiodically Induced Pharbitis nil’ Seedlings

Abstract

Keywords

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