Proteins modified with DNAzymes or aptamers act as biosensors or biosensor labels

Bella Shlyahovsky; Di Li; Eugenii Katz; Itamar Willner

doi:10.1016/j.bios.2006.10.009

Proteins modified with DNAzymes or aptamers act as biosensors or biosensor labels

Bella Shlyahovsky, Di Li, Eugenii Katz, Itamar Willner^*

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

101 Scopus citations

Abstract

Hybrid systems composed of a glucose oxidase (GOx)/peroxidase-mimicking DNAzyme, and of microperoxidase-11 (MP-11)/anti-thrombin aptamer were synthesized. The hybrid systems were employed as amplifying labels for the colorimetric or chemiluminescence detection of an enzyme functions, and thrombin analysis, respectively. In the GOx/DNAzyme system, the GOx-mediated oxidation of glucose led to the formation of H₂O₂, and this activated the oxidation of ABTS to a colored product, or to the generation of chemiluminescence in the presence of luminol. The MP-11/anti-thrombin aptamer enabled the amplified analysis of thrombin by the MP-11-mediated generation of chemiluminescence in the presence of luminol/H₂O₂.

Original language	English
Pages (from-to)	2570-2576
Number of pages	7
Journal	Biosensors and Bioelectronics
Volume	22
Issue number	11
DOIs	https://doi.org/10.1016/j.bios.2006.10.009
State	Published - 15 May 2007

Keywords

Aptamer
Biosensor
Chemiluminescence
DNAzyme
Glucose oxidase
Thrombin

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10.1016/j.bios.2006.10.009

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title = "Proteins modified with DNAzymes or aptamers act as biosensors or biosensor labels",

abstract = "Hybrid systems composed of a glucose oxidase (GOx)/peroxidase-mimicking DNAzyme, and of microperoxidase-11 (MP-11)/anti-thrombin aptamer were synthesized. The hybrid systems were employed as amplifying labels for the colorimetric or chemiluminescence detection of an enzyme functions, and thrombin analysis, respectively. In the GOx/DNAzyme system, the GOx-mediated oxidation of glucose led to the formation of H2O2, and this activated the oxidation of ABTS to a colored product, or to the generation of chemiluminescence in the presence of luminol. The MP-11/anti-thrombin aptamer enabled the amplified analysis of thrombin by the MP-11-mediated generation of chemiluminescence in the presence of luminol/H2O2.",

keywords = "Aptamer, Biosensor, Chemiluminescence, DNAzyme, Glucose oxidase, Thrombin",

author = "Bella Shlyahovsky and Di Li and Eugenii Katz and Itamar Willner",

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AU - Shlyahovsky, Bella

AU - Li, Di

AU - Katz, Eugenii

AU - Willner, Itamar

PY - 2007/5/15

Y1 - 2007/5/15

N2 - Hybrid systems composed of a glucose oxidase (GOx)/peroxidase-mimicking DNAzyme, and of microperoxidase-11 (MP-11)/anti-thrombin aptamer were synthesized. The hybrid systems were employed as amplifying labels for the colorimetric or chemiluminescence detection of an enzyme functions, and thrombin analysis, respectively. In the GOx/DNAzyme system, the GOx-mediated oxidation of glucose led to the formation of H2O2, and this activated the oxidation of ABTS to a colored product, or to the generation of chemiluminescence in the presence of luminol. The MP-11/anti-thrombin aptamer enabled the amplified analysis of thrombin by the MP-11-mediated generation of chemiluminescence in the presence of luminol/H2O2.

AB - Hybrid systems composed of a glucose oxidase (GOx)/peroxidase-mimicking DNAzyme, and of microperoxidase-11 (MP-11)/anti-thrombin aptamer were synthesized. The hybrid systems were employed as amplifying labels for the colorimetric or chemiluminescence detection of an enzyme functions, and thrombin analysis, respectively. In the GOx/DNAzyme system, the GOx-mediated oxidation of glucose led to the formation of H2O2, and this activated the oxidation of ABTS to a colored product, or to the generation of chemiluminescence in the presence of luminol. The MP-11/anti-thrombin aptamer enabled the amplified analysis of thrombin by the MP-11-mediated generation of chemiluminescence in the presence of luminol/H2O2.

KW - Aptamer

KW - Biosensor

KW - Chemiluminescence

KW - DNAzyme

KW - Glucose oxidase

KW - Thrombin

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Proteins modified with DNAzymes or aptamers act as biosensors or biosensor labels

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