Proteolysis and membrane capture of F-spondin generates combinatorial guidance cues from a single molecule

Sophie Zisman, Karen Marom, Oshri Avraham, Lilah Rinsky-Halivni, Uri Gai, Gilit Kligun, Vered Tzarfaty-Majar, Tatsuo Suzuki, Avihu Klar*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


The formation of neuronal networks is governed by a limited number of guidance molecules, yet it is immensely complex. The complexity of guidance cues is augmented by posttranslational modification of guidance molecules and their receptors. We report here that cleavage of the floor plate guidance molecule F-spondin generates two functionally opposing fragments: a shortrange repellent protein deposited in the membrane of floor plate cells and an adhesive protein that accumulates at the basement membrane. Their coordinated activity, acting respectively as a short-range repellant and a permissive short-range attractant, constricts commissural axons to the basement membrane beneath the floor plate cells. We further demonstrate that the repulsive activity of the inhibitory fragment of F-spondin requires its presentation by the lipoprotein receptor-related protein (LRP) receptors apolipoprotein E receptor 2, LRP2/megalin, and LRP4, which are expressed in the floor plate. Thus, proteolysis and membrane interaction coordinate combinatorial guidance signaling originating from a single guidance cue.

Original languageAmerican English
Pages (from-to)1237-1249
Number of pages13
JournalJournal of Cell Biology
Issue number7
StatePublished - 24 Sep 2007


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