TY - JOUR
T1 - Proteolysis of the phage λ CII regulatory protein by FtsH (HfIB) of Escherichia coli
AU - Shotland, Y.
AU - Koby, S.
AU - Teff, D.
AU - Mansur, N.
AU - Oren, D. A.
AU - Tatematsu, K.
AU - Tomoyasu, T.
AU - Kessel, M.
AU - Bukau, B.
AU - Ogura, T.
AU - Oppenheim, A. B.
PY - 1997
Y1 - 1997
N2 - Rapid proteolysis plays an important role in regulation of gene expression. Proteolysis of the phage λ CII transcriptional activator plays a key role in the lysis-lysogeny decision by phage λ. Here we demonstrate that the E. coli ATP-dependent protease FtsH, the product of the host ftsH/hflB gene, is responsible for the rapid proteolysis of the CII protein. FtsH was found previously to degrade the heat-shock transcription factor σ32. Proteolysis of σ32 requires, in vivo, the presence of the DnaK-DnaJ-GrpE chaperone machine. Neither DnaK-DnaJ-GrpE nor GroEL-GroES chaperone machines are required for proteolysis of CII in vivo. Purified Fish carries out specific ATP-dependent proteolysis of CII in vitro. The degradation of CII is at least 10-fold faster than that of σ32. Electron microscopy revealed that purified FtsH forms ring-shaped structures with a diameter of 6-7 nm.
AB - Rapid proteolysis plays an important role in regulation of gene expression. Proteolysis of the phage λ CII transcriptional activator plays a key role in the lysis-lysogeny decision by phage λ. Here we demonstrate that the E. coli ATP-dependent protease FtsH, the product of the host ftsH/hflB gene, is responsible for the rapid proteolysis of the CII protein. FtsH was found previously to degrade the heat-shock transcription factor σ32. Proteolysis of σ32 requires, in vivo, the presence of the DnaK-DnaJ-GrpE chaperone machine. Neither DnaK-DnaJ-GrpE nor GroEL-GroES chaperone machines are required for proteolysis of CII in vivo. Purified Fish carries out specific ATP-dependent proteolysis of CII in vitro. The degradation of CII is at least 10-fold faster than that of σ32. Electron microscopy revealed that purified FtsH forms ring-shaped structures with a diameter of 6-7 nm.
UR - http://www.scopus.com/inward/record.url?scp=8544283778&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2958.1997.4231796.x
DO - 10.1046/j.1365-2958.1997.4231796.x
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C2 - 9218777
AN - SCOPUS:8544283778
SN - 0950-382X
VL - 24
SP - 1303
EP - 1310
JO - Molecular Microbiology
JF - Molecular Microbiology
IS - 6
ER -