Proteomic analysis of human natural killer cells: Insights on new potential NK immune functions

Jacob Hanna, Jonathan Fitchett, Tony Rowe, Mark Daniels, Melissa Heller, Tsufit Gonen-Gross, Efrat Manaster, Samuel Y. Cho, Michael J. Labarre, Ofer Mandelboim

Research output: Contribution to journalShort surveypeer-review

24 Scopus citations

Abstract

Applying high-throughput proteomic analysis of mammalian cells can facilitate the identification of a large number of proteins expressed in the examined samples. Moreover, extensive research efforts are being made to perform large-scale characterization of membrane proteins. Here we use mass spectrometry-based proteomic strategy to characterize protein expression in membrane-enriched fractions derived from human NK lymphoma cell line YTS. This query yielded a list of over 1000 identified proteins, and provided us with new insights on NK cell biology. We highlight the expression of CD86 on YTS and its ability to co-stimulate TCR responses of human CD4+ T-cells, providing an unexpected link between innate and adaptive immune systems.

Original languageAmerican English
Pages (from-to)425-431
Number of pages7
JournalMolecular Immunology
Volume42
Issue number4 SPEC. ISS.
DOIs
StatePublished - Feb 2005

Bibliographical note

Funding Information:
We thank Nabil Hanna for helpful ideas and discussions. We also thank Lisa Berquist, Judith Giri, Hari Hariharan, Loic Scales, Annie Glidden for excellent assistance. O. Mandelboim is supported by research grants from the Israel Cancer Research Foundation, The Israel Science Foundation, European Commission (QLK2-CT-2002-011112) and the Israeli Cancer Research Institute.

Keywords

  • Mass spectrometry
  • NK
  • Proteomics

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