Proteomic analysis of protein components in periodontal ligament fibroblasts

E. Reichenberg, M. Redlich, P. Cancemi, B. Zaks, S. Pitaru, S. Fontana, I. Pucci-Minafra, A. Palmon*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


Background: Characterization of periodontal ligament (PDL) fibroblast proteome is an important tool for understanding PDL physiology and regulation and for identifying disease-related protein markers. PDL fibroblast protein expression has been studied using immunological methods, although limited to previously identified proteins for which specific antibodies are available. Methods: We applied proteomic analysis coupled with mass spectrometry and database knowledge to human PDL fibroblasts. Results: We detected 900 spots and identified 117 protein spots originating in 74 different genes. In addition to scaffold cytoskeletal proteins, e.g., actin, tubulin, and vimentin, we identified proteins implicated with cellular motility and membrane trafficking, chaparonine, stress and folding proteins, metabolic enzymes, proteins associated with detoxification and membrane activity, biodegradative metabolism, translation and transduction, extracellular proteins, and cell cycle regulation proteins. Conclusions: Most of these identified proteins are closely related to the extensive PDL fibroblasts' functions and homeostasis. Our PDL fibroblast proteome map can serve as a reference map for future clinical studies as well as basic research.

Original languageAmerican English
Pages (from-to)1645-1653
Number of pages9
JournalJournal of Periodontology
Issue number10
StatePublished - Oct 2005


  • Fibroblasts
  • Periodontal ligament
  • Proteome
  • Proteomics


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