Abstract
The accessibility of different chromatin regions to transcription factors and other DNA-binding proteins is a critical determinant of cell function. Here, we detail a modified assay for transposase-accessible chromatin sequencing (ATAC-seq) protocol which measures chromatin accessibility genome wide. We describe nuclei isolation, tagmentation, PCR amplification, and pre- and post-sequencing quality control. Our protocol is optimized for the liver, a tissue where nuclei isolation requires distinct steps. We provide two detailed vignettes: one for bulk ATAC-seq and another for single-nuclei ATAC-seq.
Original language | English |
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Article number | 102462 |
Journal | STAR Protocols |
Volume | 4 |
Issue number | 3 |
DOIs | |
State | Published - 15 Sep 2023 |
Bibliographical note
Publisher Copyright:© 2023 The Author(s)
Keywords
- Genomics
- Metabolism
- Molecular Biology
- Single Cell