Protocol for parotidectomy and saliva analysis in mice

Khaled Zubeidat; Yasmin Saba; Or Barel; Fady L. Shoukair; Avi Hai Hovav

doi:10.1016/j.xpro.2021.101048

Protocol for parotidectomy and saliva analysis in mice

Khaled Zubeidat^*, Yasmin Saba, Or Barel, Fady L. Shoukair, Avi Hai Hovav^*

^*Corresponding author for this work

Institute of Biomedical Research at the Faculty of Dental Medicine

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The murine parotid salivary glands develop postnatally, shaping oral mucosal immunity in early and adult life. This protocol details the surgical removal of the parotid glands (parotidectomy) of mice. We also describe a protocol for saliva collection to enable manipulation and measurement of physiological and immunological salivary functions. Our saliva collection approach has been modified from published protocols to enable saliva collection from young mice, which can be challenging. For complete details on the use and execution of this protocol, please refer to Koren et al. (2020).

Original language	English
Article number	101048
Journal	STAR Protocols
Volume	3
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2021.101048
State	Published - 18 Mar 2022

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Publisher Copyright:
© 2021 The Authors

Keywords

Immunology
Microbiology
Model Organisms

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10.1016/j.xpro.2021.101048

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AB - The murine parotid salivary glands develop postnatally, shaping oral mucosal immunity in early and adult life. This protocol details the surgical removal of the parotid glands (parotidectomy) of mice. We also describe a protocol for saliva collection to enable manipulation and measurement of physiological and immunological salivary functions. Our saliva collection approach has been modified from published protocols to enable saliva collection from young mice, which can be challenging. For complete details on the use and execution of this protocol, please refer to Koren et al. (2020).

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Protocol for parotidectomy and saliva analysis in mice

Abstract

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