Protocol for phage matching, treatment, and monitoring for compassionate bacteriophage use in non-resolving infections

Hadil Onallah; Ortal Yerushalmy; Ron Braunstein; Sivan Alkalay-Oren; Amit Rimon; Daniel Gelman; Shunit Coppenhagen-Glazer; Ronen Hazan; Ran Nir-Paz

doi:10.1016/j.xpro.2024.102949

Protocol for phage matching, treatment, and monitoring for compassionate bacteriophage use in non-resolving infections

Hadil Onallah, Ortal Yerushalmy, Ron Braunstein, Sivan Alkalay-Oren, Amit Rimon, Daniel Gelman, Shunit Coppenhagen-Glazer, Ronen Hazan^*, Ran Nir-Paz^*

^*Corresponding author for this work

Institute of Biomedical Research at the Faculty of Dental Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Phage therapy has re-emerged as a promising treatment for non-resolving infections. Given the lack of approved phage treatments, there is a need to establish a compassionate use pipeline. Here, we present a protocol for phage matching, treatment, and monitoring for compassionate bacteriophage use in non-resolving infections. We describe steps for consultation and request implementation, evaluating and comparing different aspects of phage activity, and phage production. We then detail procedures for multidisciplinary meetings, ethics approvals, phage therapy, and follow-up. For complete details on the use and execution of this protocol, please refer to Onallah et al.¹^,²

Original language	English
Article number	102949
Journal	STAR Protocols
Volume	5
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2024.102949
State	Published - 21 Jun 2024

Bibliographical note

Publisher Copyright:
© 2024 The Authors

Keywords

Clinical Protocol
Health Sciences
Microbiology

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10.1016/j.xpro.2024.102949

Cite this

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AU - Braunstein, Ron

AU - Alkalay-Oren, Sivan

AU - Rimon, Amit

AU - Gelman, Daniel

AU - Coppenhagen-Glazer, Shunit

AU - Hazan, Ronen

AU - Nir-Paz, Ran

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Protocol for phage matching, treatment, and monitoring for compassionate bacteriophage use in non-resolving infections

Abstract

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