Protocol for Primary Mouse Hepatocyte Isolation

Meital Charni-Natan, Ido Goldstein*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

141 Scopus citations

Abstract

Primary hepatocytes are a vital tool in various biomedical research disciplines, serving as an ex vivo model for liver physiology. Obtaining high yields of viable primary mouse hepatocytes is technically challenging, limiting their use. Here, we present an improved protocol based on the classic two-step collagenase perfusion technique. The liver is washed by perfusion, hepatocytes are dissociated by collagenase, separated from other cells, and cultured. This protocol was optimized to significantly reduce procedure duration and improve hepatocyte yield and viability.

Original languageAmerican English
Article number100086
JournalSTAR Protocols
Volume1
Issue number2
DOIs
StatePublished - 18 Sep 2020

Bibliographical note

Publisher Copyright:
© 2020 The Author(s)

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