Pumping iron: A multi-omics analysis of two extremophilic algae reveals iron economy management

Lital Davidi, Sean D. Gallaher*, Eyal Ben-David, Samuel O. Purvine, Thomas L. Fillmore, Carrie D. Nicora, Rory J. Craig, Stefan Schmollinger, Sanja Roje, Crysten E. Blaby-Haas, Robert P. Auber, Jennifer H. Wisecaver, Sabeeha S. Merchant*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Marine algae are responsible for half of the world's primary productivity, but this critical carbon sink is often constrained by insufficient iron. One species of marine algae, Dunaliella tertiolecta, is remarkable for its ability to maintain photosynthesis and thrive in low-iron environments. A related species, Dunaliella salina Bardawil, shares this attribute but is an extremophile found in hypersaline environments. To elucidate how algae manage their iron requirements, we produced high-quality genome assemblies and transcriptomes for both species to serve as a foundation for a comparative multiomics analysis. We identified a host of iron-uptake proteins in both species, including a massive expansion of transferrins and a unique family of siderophore-iron-uptake proteins. Complementing these multiple iron-uptake routes, ferredoxin functions as a large iron reservoir that can be released by induction of flavodoxin. Proteomic analysis revealed reduced investment in the photosynthetic apparatus coupled with remodeling of antenna proteins by dramatic iron-deficiency induction of TIDI1, which is closely related but identifiably distinct from the chlorophyll binding protein, LHCA3. These combinatorial iron scavenging and sparing strategies make Dunaliella unique among photosynthetic organisms.

Original languageAmerican English
Article numbere2305495120
JournalProceedings of the National Academy of Sciences of the United States of America
Volume120
Issue number30
DOIs
StatePublished - 25 Jul 2023
Externally publishedYes

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