Purification and composition of photosystem I reaction center of Prochloron sp., an oxygen-evolving prokaryote containing chlorophyll b

Gadi Schuster; Rachel Nechushtai; Nathan Nelson; Itzhak Ohad

doi:10.1016/0014-5793(85)80987-X

Purification and composition of photosystem I reaction center of Prochloron sp., an oxygen-evolving prokaryote containing chlorophyll b

Gadi Schuster, Rachel Nechushtai, Nathan Nelson, Itzhak Ohad^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The photosystem I reaction center complex of the photosynthetic prokaryote Prochloron, present as a symbiont in ascidians from the Red Sea at Eilat (Israel), was isolated and characterized. The complex consists of 4 polypeptide subunits, and therefore is similar to that of cyanophytes and green algae. Their apparent molecular masses (in kDa) are about 70 (subunit I), 16 (subunit II), 10 (subunit III), and 8 (subunit IV). The purified reaction center contains about 40 chlorophyll molecules per P-700 as compared to about 800 in the intact thylakoids. Subunit I has the same apparent electrophoretic mobility and appears as a double polypeptide band as reported for this subunit in higher plants and algae. Immunological cross-reactivity was detected among subunits I and II of Prochloron and photosystem I reaction centers of higher plants and algae.

Original language	English
Pages (from-to)	29-33
Number of pages	5
Journal	FEBS Letters
Volume	191
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(85)80987-X
State	Published - 21 Oct 1985
Externally published	Yes

Keywords

P-700
Photosystem I
Prochloron
Reaction center submit

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10.1016/0014-5793(85)80987-X

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title = "Purification and composition of photosystem I reaction center of Prochloron sp., an oxygen-evolving prokaryote containing chlorophyll b",

abstract = "The photosystem I reaction center complex of the photosynthetic prokaryote Prochloron, present as a symbiont in ascidians from the Red Sea at Eilat (Israel), was isolated and characterized. The complex consists of 4 polypeptide subunits, and therefore is similar to that of cyanophytes and green algae. Their apparent molecular masses (in kDa) are about 70 (subunit I), 16 (subunit II), 10 (subunit III), and 8 (subunit IV). The purified reaction center contains about 40 chlorophyll molecules per P-700 as compared to about 800 in the intact thylakoids. Subunit I has the same apparent electrophoretic mobility and appears as a double polypeptide band as reported for this subunit in higher plants and algae. Immunological cross-reactivity was detected among subunits I and II of Prochloron and photosystem I reaction centers of higher plants and algae.",

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AU - Nechushtai, Rachel

AU - Nelson, Nathan

AU - Ohad, Itzhak

PY - 1985/10/21

Y1 - 1985/10/21

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AB - The photosystem I reaction center complex of the photosynthetic prokaryote Prochloron, present as a symbiont in ascidians from the Red Sea at Eilat (Israel), was isolated and characterized. The complex consists of 4 polypeptide subunits, and therefore is similar to that of cyanophytes and green algae. Their apparent molecular masses (in kDa) are about 70 (subunit I), 16 (subunit II), 10 (subunit III), and 8 (subunit IV). The purified reaction center contains about 40 chlorophyll molecules per P-700 as compared to about 800 in the intact thylakoids. Subunit I has the same apparent electrophoretic mobility and appears as a double polypeptide band as reported for this subunit in higher plants and algae. Immunological cross-reactivity was detected among subunits I and II of Prochloron and photosystem I reaction centers of higher plants and algae.

KW - P-700

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Purification and composition of photosystem I reaction center of Prochloron sp., an oxygen-evolving prokaryote containing chlorophyll b

Abstract

Keywords

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