TY - JOUR
T1 - Purification and reconstitution of the phosphate transporter from bovine heart mitochondria
AU - Banerjee, Ranajit K.
AU - Shertzer, Howard G.
AU - Kanner, Baruch I.
AU - Racker, Efraim
PY - 1977/4/11
Y1 - 1977/4/11
N2 - Phosphate transporter of bovine heart mitochondria was purified by solubilization of submitochondrial particles with octylglucoside and fractionation of the extract with ammonium sulfate. After reconstitution into liposomes the purified protein catalyzed phosphate transport which was sensitive to mersalyl and other SH reagents. Transport measured either as Pi OH or Pi Pi exchange was proportional to protein concentration and time. The Pi OH but not the Pi Pi exchange was stimulated several fold by valinomycin plus nigericin in the presence of K+. The reconstituted system provides a suitable assay during purification of the mitochondrial phosphate transporter.
AB - Phosphate transporter of bovine heart mitochondria was purified by solubilization of submitochondrial particles with octylglucoside and fractionation of the extract with ammonium sulfate. After reconstitution into liposomes the purified protein catalyzed phosphate transport which was sensitive to mersalyl and other SH reagents. Transport measured either as Pi OH or Pi Pi exchange was proportional to protein concentration and time. The Pi OH but not the Pi Pi exchange was stimulated several fold by valinomycin plus nigericin in the presence of K+. The reconstituted system provides a suitable assay during purification of the mitochondrial phosphate transporter.
UR - http://www.scopus.com/inward/record.url?scp=0017332114&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(77)91539-X
DO - 10.1016/0006-291X(77)91539-X
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C2 - 856184
AN - SCOPUS:0017332114
SN - 0006-291X
VL - 75
SP - 772
EP - 778
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -