Purification of peptidyl-tRNA on benzoylated DEAE-cellulose columns

Don Eilat; Ehud Ziv; Yehuda Lapidot

doi:10.1093/nar/2.12.2237

Purification of peptidyl-tRNA on benzoylated DEAE-cellulose columns

Don Eilat^*
, Ehud Ziv
, Yehuda Lapidot

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The o-nitrophenylsulfenyl group, an amino protecting group in the chemical synthesis of peptidyl-tRNA was used to attach newly synthesized peptidyl-tRNA to a benzoylated DEAE-cellulose column. This facilitated the isolation of a highly purified specific tRNA with a well defined peptide chain.

Original language	English
Pages (from-to)	2237-2250
Number of pages	14
Journal	Nucleic Acids Research
Volume	2
Issue number	12
DOIs	https://doi.org/10.1093/nar/2.12.2237
State	Published - Dec 1975

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title = "Purification of peptidyl-tRNA on benzoylated DEAE-cellulose columns",

abstract = "The o-nitrophenylsulfenyl group, an amino protecting group in the chemical synthesis of peptidyl-tRNA was used to attach newly synthesized peptidyl-tRNA to a benzoylated DEAE-cellulose column. This facilitated the isolation of a highly purified specific tRNA with a well defined peptide chain.",

author = "Don Eilat and Ehud Ziv and Yehuda Lapidot",

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AB - The o-nitrophenylsulfenyl group, an amino protecting group in the chemical synthesis of peptidyl-tRNA was used to attach newly synthesized peptidyl-tRNA to a benzoylated DEAE-cellulose column. This facilitated the isolation of a highly purified specific tRNA with a well defined peptide chain.

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Purification of peptidyl-tRNA on benzoylated DEAE-cellulose columns

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