TY - JOUR
T1 - Purification of two Leishmania donovani membrane proteins recognized by sera from patients with visceral leishmaniasis
AU - Jaffe, Charles L.
AU - Zalis, Mariano
PY - 1988/1/1
Y1 - 1988/1/1
N2 - Two Leishmania donovani membrane proteins recognized by sera from patients with visceral leishmaniasis were purified using species-specific monoclonal antibodies and characterized. The molecular weights of the proteins, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, were ∼ 70 000 and ∼ 72 000, respectively. The 70 kDa protein, which appears as a diffuse band on silver staining, was resolved into a doublet by Western blotting with monoclonal antibody. Though of similar molecular weight and amino acid composition, the two proteins were shown to be distinct by peptide mapping and Western blotting of the purified material. The two proteins are recognized specifically by human visceral leishmaniasis serum and not by serum from cutaneous leishmaniasis or Chagas' disease. These proteins will be useful in developing a direct serodiagnostic assay for visceral leishmaniasis.
AB - Two Leishmania donovani membrane proteins recognized by sera from patients with visceral leishmaniasis were purified using species-specific monoclonal antibodies and characterized. The molecular weights of the proteins, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, were ∼ 70 000 and ∼ 72 000, respectively. The 70 kDa protein, which appears as a diffuse band on silver staining, was resolved into a doublet by Western blotting with monoclonal antibody. Though of similar molecular weight and amino acid composition, the two proteins were shown to be distinct by peptide mapping and Western blotting of the purified material. The two proteins are recognized specifically by human visceral leishmaniasis serum and not by serum from cutaneous leishmaniasis or Chagas' disease. These proteins will be useful in developing a direct serodiagnostic assay for visceral leishmaniasis.
KW - Immunodiagnosis
KW - Leishmania donovani
KW - Membrane protein purification
KW - Patient sera
UR - http://www.scopus.com/inward/record.url?scp=0023640935&partnerID=8YFLogxK
U2 - 10.1016/0166-6851(88)90024-2
DO - 10.1016/0166-6851(88)90024-2
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C2 - 3343999
AN - SCOPUS:0023640935
SN - 0166-6851
VL - 27
SP - 53
EP - 62
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 1
ER -