Pyrokinin/PBAN radio-receptor assay: Development and application for the characterization of a putative receptor from the pheromone gland of Heliothis peltigera

Miriam Altstein; Orna Ben-Aziz; Shai Daniel; Irina Zeltser; Chaim Gilon

doi:10.1016/S0196-9781(01)00479-X

Pyrokinin/PBAN radio-receptor assay: Development and application for the characterization of a putative receptor from the pheromone gland of Heliothis peltigera

Miriam Altstein^*, Orna Ben-Aziz, Shai Daniel, Irina Zeltser, Chaim Gilon

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

A radio-receptor assay (RRA) for the insect pyrokinin/PBAN family has been developed. The development involved examination of the ligand (³H-tyrosyl-PBAN28-33NH₂)-receptor interaction under various incubation conditions and variations on sex pheromone gland membrane preparation. Application of the RRA for a partial characterization of the putative pyrokinin/PBAN receptor in the pheromone gland of H. peltigera revealed age-dependence of its expression. Pharmacological characterization revealed a high correlation between the binding-affinity to the receptor of various PBAN-derived peptides and their in vivo pheromonotropic bioactivity, and shed light on the interaction of backbone cyclic and linear ([Arg²⁷,D-Phe³⁰]PBAN28-33NH₂) PBAN antagonists with the receptor.

Original language	English
Pages (from-to)	1379-1389
Number of pages	11
Journal	Peptides
Volume	22
Issue number	9
DOIs	https://doi.org/10.1016/S0196-9781(01)00479-X
State	Published - 2001

Keywords

Heliothis peltigera
Insect neuropeptide
Moths
PBAN
PBAN receptor
Pheromone gland
Radio receptor assay

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10.1016/S0196-9781(01)00479-X

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title = "Pyrokinin/PBAN radio-receptor assay: Development and application for the characterization of a putative receptor from the pheromone gland of Heliothis peltigera",

abstract = "A radio-receptor assay (RRA) for the insect pyrokinin/PBAN family has been developed. The development involved examination of the ligand (3H-tyrosyl-PBAN28-33NH2)-receptor interaction under various incubation conditions and variations on sex pheromone gland membrane preparation. Application of the RRA for a partial characterization of the putative pyrokinin/PBAN receptor in the pheromone gland of H. peltigera revealed age-dependence of its expression. Pharmacological characterization revealed a high correlation between the binding-affinity to the receptor of various PBAN-derived peptides and their in vivo pheromonotropic bioactivity, and shed light on the interaction of backbone cyclic and linear ([Arg27,D-Phe30]PBAN28-33NH2) PBAN antagonists with the receptor.",

keywords = "Heliothis peltigera, Insect neuropeptide, Moths, PBAN, PBAN receptor, Pheromone gland, Radio receptor assay",

author = "Miriam Altstein and Orna Ben-Aziz and Shai Daniel and Irina Zeltser and Chaim Gilon",

year = "2001",

doi = "10.1016/S0196-9781(01)00479-X",

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T2 - Development and application for the characterization of a putative receptor from the pheromone gland of Heliothis peltigera

AU - Altstein, Miriam

AU - Ben-Aziz, Orna

AU - Daniel, Shai

AU - Zeltser, Irina

AU - Gilon, Chaim

PY - 2001

Y1 - 2001

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AB - A radio-receptor assay (RRA) for the insect pyrokinin/PBAN family has been developed. The development involved examination of the ligand (3H-tyrosyl-PBAN28-33NH2)-receptor interaction under various incubation conditions and variations on sex pheromone gland membrane preparation. Application of the RRA for a partial characterization of the putative pyrokinin/PBAN receptor in the pheromone gland of H. peltigera revealed age-dependence of its expression. Pharmacological characterization revealed a high correlation between the binding-affinity to the receptor of various PBAN-derived peptides and their in vivo pheromonotropic bioactivity, and shed light on the interaction of backbone cyclic and linear ([Arg27,D-Phe30]PBAN28-33NH2) PBAN antagonists with the receptor.

KW - Heliothis peltigera

KW - Insect neuropeptide

KW - Moths

KW - PBAN

KW - PBAN receptor

KW - Pheromone gland

KW - Radio receptor assay

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ER -

Pyrokinin/PBAN radio-receptor assay: Development and application for the characterization of a putative receptor from the pheromone gland of Heliothis peltigera

Abstract

Keywords

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