Quantitative computerized western blotting in detail

Dalit Talmi-Frank*, Charles L. Jaffe, Gad Baneth

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

The analysis of antibody reactivity against multiple antigens separated according to their molecular weights is facilitated by western blotting. The distinction between immune dominant and recessive antigens is often difficult and carried out by qualitative or empirical means. Quantitative computerized western blotting (QCWB) analyzes reactivity to specific antigens by providing a statistically measurable value for each band allowing differentiation between immunodominant and immunorecessive determinants. QCWB is useful for both single time point analysis and longitudinal studies where multiple time points are evaluated and the relativities against individual bands compared. This technique can be employed to study humoral responses to complex antigenic mixtures such as allergens and infectious agents, or identify serologic markers for early diagnosis of cancer, autoimmune or infectious diseases, or to monitor patient's clinical status.

Original languageAmerican English
Title of host publicationWestern Blotting
Subtitle of host publicationMethods and Protocols
PublisherSpringer New York
Pages141-150
Number of pages10
ISBN (Electronic)9781493926947
ISBN (Print)9781493926930
DOIs
StatePublished - 4 Jun 2015
Externally publishedYes

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015. All right reserved.

Keywords

  • Immunodominant antigen
  • Net intensity
  • SDS-PAGE
  • Total lane intensity
  • Western blot, quantitative computerized western blot

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