Quantitative measurements of type I and type II persisters using ScanLag

Irit Levin-Reisman, Nathalie Q. Balaban

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

14 Scopus citations

Abstract

The present method quantifies the number of slow-growing bacteria leading to antibiotic persistence in a clonal population. First, it enables discriminating between slow growers that are generated by exposure to a stress signal (Type I persisters) and slow growers that are continuously generated during exponential growth (Type II persisters). Second, the method enables determining the amount of slow growers in a culture.

Original languageAmerican English
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages75-81
Number of pages7
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1333
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2016.

Keywords

  • Automatic imaging
  • Bacterial growth
  • High-throughput measurements
  • Lag
  • Persisters
  • ScanLag
  • Stationary phase
  • Type I persistence
  • Type II persistence

Fingerprint

Dive into the research topics of 'Quantitative measurements of type I and type II persisters using ScanLag'. Together they form a unique fingerprint.

Cite this