Quantitative measurements of type I and type II persisters using ScanLag

Irit Levin-Reisman; Nathalie Q. Balaban

doi:10.1007/978-1-4939-2854-5_7

Quantitative measurements of type I and type II persisters using ScanLag

Irit Levin-Reisman, Nathalie Q. Balaban

Racah Institute of Physics

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

16 Scopus citations

Abstract

The present method quantifies the number of slow-growing bacteria leading to antibiotic persistence in a clonal population. First, it enables discriminating between slow growers that are generated by exposure to a stress signal (Type I persisters) and slow growers that are continuously generated during exponential growth (Type II persisters). Second, the method enables determining the amount of slow growers in a culture.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	75-81
Number of pages	7
DOIs	https://doi.org/10.1007/978-1-4939-2854-5_7
State	Published - 2016

Publication series

Name	Methods in Molecular Biology
Volume	1333
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2016.

Keywords

Automatic imaging
Bacterial growth
High-throughput measurements
Lag
Persisters
ScanLag
Stationary phase
Type I persistence
Type II persistence

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10.1007/978-1-4939-2854-5_7

Cite this

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KW - Persisters

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KW - Stationary phase

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KW - Type II persistence

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Quantitative measurements of type I and type II persisters using ScanLag

Abstract

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Keywords

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