Quaternary ammonium polysaccharides for gene delivery

Ira Yudovin-Farber; Chava Yanay; Tony Azzam; Michal Linial; Abraham J. Domb

doi:10.1021/bc050066p

Quaternary ammonium polysaccharides for gene delivery

Ira Yudovin-Farber, Chava Yanay, Tony Azzam, Michal Linial, Abraham J. Domb^*

^*Corresponding author for this work

Department of Biological Chemistry

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

Cationic polysaccharides were synthesized by conjugation of various monoquaternary (MQ) ammonium oligoamines to oxidized dextran by reductive amination and tested for gene transfection. Polycations of dextran grafted with MQ ammonium oligoamines of two to four amino groups were investigated for their ability to cause pCMV-GFP encoding for green fluorescence protein and β-Gal encoding for β-galactosidase protein transfection on EPC and HEK-293 cell lines. These polycations were expected to strongly complex DNA due to increased surface cationic charge of the carrier, which may result in a higher transfection yield. However, the transfection yields were much lower compared to the parent vector, dextran-spermine conjugate, which was highly effective both in vitro and in vivo.

Original language	English
Pages (from-to)	1196-1203
Number of pages	8
Journal	Bioconjugate Chemistry
Volume	16
Issue number	5
DOIs	https://doi.org/10.1021/bc050066p
State	Published - 2005

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AU - Domb, Abraham J.

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AB - Cationic polysaccharides were synthesized by conjugation of various monoquaternary (MQ) ammonium oligoamines to oxidized dextran by reductive amination and tested for gene transfection. Polycations of dextran grafted with MQ ammonium oligoamines of two to four amino groups were investigated for their ability to cause pCMV-GFP encoding for green fluorescence protein and β-Gal encoding for β-galactosidase protein transfection on EPC and HEK-293 cell lines. These polycations were expected to strongly complex DNA due to increased surface cationic charge of the carrier, which may result in a higher transfection yield. However, the transfection yields were much lower compared to the parent vector, dextran-spermine conjugate, which was highly effective both in vitro and in vivo.

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Quaternary ammonium polysaccharides for gene delivery

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