Raalin, a transcript enriched in the honey bee brain, is a remnant of genomic rearrangement in hymenoptera

Y. Tirosh, N. Morpurgo, M. Cohen, M. Linial*, G. Bloch

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

We identified a predicted compact cysteine-rich sequence in the honey bee genome that we called 'Raalin'. Raalin transcripts are enriched in the brain of adult honey bee workers and drones, with only minimum expression in other tissues or in pre-adult stages. Open-reading frame (ORF) homologues of Raalin were identified in the transcriptomes of fruit flies, mosquitoes and moths. The Raalin-like gene from Drosophila melanogaster encodes for a short secreted protein that is maximally expressed in the adult brain with negligible expression in other tissues or pre-imaginal stages. Raalin-like sequences have also been found in the recently sequenced genomes of six ant species, but not in the jewel wasp Nasonia vitripennis. As in the honey bee, the Raalin-like sequences of ants do not have an ORF. A comparison of the genome region containing Raalin in the genomes of bees, ants and the wasp provides evolutionary support for an extensive genome rearrangement in this sequence. Our analyses identify a new family of ancient cysteine-rich short sequences in insects in which insertions and genome rearrangements may have disrupted this locus in the branch leading to the Hymenoptera. The regulated expression of this transcript suggests that it has a brain-specific function.

Original languageAmerican English
Pages (from-to)305-318
Number of pages14
JournalInsect Molecular Biology
Volume21
Issue number3
DOIs
StatePublished - Jun 2012

Keywords

  • Apis mellifera
  • cysteine-rich peptide
  • genomic rearrangement
  • non-coding RNA
  • pseudogene
  • short proteins

Fingerprint

Dive into the research topics of 'Raalin, a transcript enriched in the honey bee brain, is a remnant of genomic rearrangement in hymenoptera'. Together they form a unique fingerprint.

Cite this