TY - JOUR
T1 - Radioimmunoassay for detection of antigen and antibodies to Newcastle disease virus
AU - Spira, G.
AU - Silvian, I.
AU - Zakay Rones, Z.
PY - 1976
Y1 - 1976
N2 - When Newcastle disease virus (NDV) is treated with NP 40 and ether a membrane fraction of 150,000 m.w. is obtained. This fraction, which is composed of two polypeptides with m.w. of 56,000 and 76,000, was used in a radioimmunoassay (RIA). The assay was developed for both antigen and antibody and was found to be reproducible, specific, and highly sensitive. Titers of 1:51,200 were determined by RIA as compared to 1:4 by agar gel diffusion and 1:200 by hemagglutination inhibition (HI). As little as 5 ng of viral protein were detected by RIA inhibition technique. Labeled antigen could be stored in the presence of serum, KCl and Titon X 100 at -20°C for as long as 6 weeks and retained similar reactivity as fresh reagent.
AB - When Newcastle disease virus (NDV) is treated with NP 40 and ether a membrane fraction of 150,000 m.w. is obtained. This fraction, which is composed of two polypeptides with m.w. of 56,000 and 76,000, was used in a radioimmunoassay (RIA). The assay was developed for both antigen and antibody and was found to be reproducible, specific, and highly sensitive. Titers of 1:51,200 were determined by RIA as compared to 1:4 by agar gel diffusion and 1:200 by hemagglutination inhibition (HI). As little as 5 ng of viral protein were detected by RIA inhibition technique. Labeled antigen could be stored in the presence of serum, KCl and Titon X 100 at -20°C for as long as 6 weeks and retained similar reactivity as fresh reagent.
UR - http://www.scopus.com/inward/record.url?scp=0017105502&partnerID=8YFLogxK
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C2 - 1254960
AN - SCOPUS:0017105502
SN - 0022-1767
VL - 116
SP - 1089
EP - 1092
JO - Journal of Immunology
JF - Journal of Immunology
IS - 4
ER -