Raman spectrometric discrimination of flexirubin pigments from two genera of Bacteroidetes

Jan Jehlička; Kateřina Osterrothová; Aharon Oren; Howell G.M. Edwards

doi:10.1111/1574-6968.12243

Raman spectrometric discrimination of flexirubin pigments from two genera of Bacteroidetes

Jan Jehlička^*, Kateřina Osterrothová, Aharon Oren, Howell G.M. Edwards

^*Corresponding author for this work

Department of Plant and Environmental Sciences (Natural Sciences)

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Flexirubins are specific polyene pigments produced by several genera of Bacteroidetes. Colonies and cell extracts of Flavobacterium johnsoniae and Flexibacter elegans have been investigated by Raman spectroscopy to show that this fast and non-destructive technique can be used to differentiate these pigments from carotenoids and to compare the flexirubin content of the two microorganisms. The presence or absence of certain distinguishing features in the CH combination band region at 2500-2750 cm^-1 can assist in the discrimination between the two flexirubins investigated. Raman spectroscopy is thus a suitable tool not only to detect flexirubin pigments in bacterial cells, but also to further characterize the pigments present in members of the Bacteroidetes genera that are rich in flexirubins.

Original language	English
Pages (from-to)	97-102
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	348
Issue number	2
DOIs	https://doi.org/10.1111/1574-6968.12243
State	Published - Nov 2013

Keywords

Chemotaxonomy
Dialkylated resorcinol
Identification
p-hydroxyphenyl group

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10.1111/1574-6968.12243

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abstract = "Flexirubins are specific polyene pigments produced by several genera of Bacteroidetes. Colonies and cell extracts of Flavobacterium johnsoniae and Flexibacter elegans have been investigated by Raman spectroscopy to show that this fast and non-destructive technique can be used to differentiate these pigments from carotenoids and to compare the flexirubin content of the two microorganisms. The presence or absence of certain distinguishing features in the CH combination band region at 2500-2750 cm-1 can assist in the discrimination between the two flexirubins investigated. Raman spectroscopy is thus a suitable tool not only to detect flexirubin pigments in bacterial cells, but also to further characterize the pigments present in members of the Bacteroidetes genera that are rich in flexirubins.",

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Raman spectrometric discrimination of flexirubin pigments from two genera of Bacteroidetes

Abstract

Keywords

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