Random mixtures of antimicrobial peptides inhibit bacteria associated with pasteurized bovine milk

Tal Stern Bauer; Zvi Hayouka

doi:10.1002/psc.3088

Random mixtures of antimicrobial peptides inhibit bacteria associated with pasteurized bovine milk

Tal Stern Bauer, Zvi Hayouka^*

^*Corresponding author for this work

Institute of Biochemistry, Food Science and Nutrition

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The shelf life of pasteurized bovine milk is limited by microorganism activity as surviving bacteria continue to grow in the bovine milk, eventually causing milk spoilage. In the current study, we used matrix-assisted laser desorption ionization time of flight mass spectrometry to identify pasteurized bovine milk-associated mesophilic and psychrotrophic bacteria. We have recently designed random cationic peptide mixtures that possess strong antimicrobial and antibiofilm properties. These compounds are cheap and easy to synthesize and represent a new class of antimicrobial agents. Here, we show that the random peptide mixtures are able to efficiently eradicate the bacteria identified as associated with pasteurized bovine milk, and reduced significantly the growth of Bacillus subtilis in milk. We propose these antimicrobial peptides as potential candidates for integration in bioactive milk and food packaging to prevent bacterial growth and extend the shelf life of food.

Original language	American English
Article number	e3088
Journal	Journal of Peptide Science
Volume	24
Issue number	7
DOIs	https://doi.org/10.1002/psc.3088
State	Published - Jul 2018

Bibliographical note

Funding Information:
This work was supported by the Israel Ministry of Health for research projects and fellowship fund on food and nutrition with implications on public health (grant number 3-11626) and by the Research Center for Nutrigenomics and Functional Food of the Hebrew University of Jerusalem. The authors would like to thank Dr. Roni Shapira for fruitful discussions and suggestions, Itamar Ilia from Bruker Israel for his professional technical support and kindness, and Dr. Irena Peri for reading the manuscript.

Funding Information:
This work was supported by the Israel Ministry of Health for research projects and fellowship fund on food and nutrition with implications on public health (grant number 3‐11626) and by the Research Center for Nutrigenomics and Functional Food of the Hebrew University of Jerusalem.

Publisher Copyright:
Copyright © 2018 European Peptide Society and John Wiley & Sons, Ltd.

Keywords

MALDI-TOF MS
antimicrobial peptide
bioactive packaging
bovine milk spoilage bacteria

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10.1002/psc.3088

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abstract = "The shelf life of pasteurized bovine milk is limited by microorganism activity as surviving bacteria continue to grow in the bovine milk, eventually causing milk spoilage. In the current study, we used matrix-assisted laser desorption ionization time of flight mass spectrometry to identify pasteurized bovine milk-associated mesophilic and psychrotrophic bacteria. We have recently designed random cationic peptide mixtures that possess strong antimicrobial and antibiofilm properties. These compounds are cheap and easy to synthesize and represent a new class of antimicrobial agents. Here, we show that the random peptide mixtures are able to efficiently eradicate the bacteria identified as associated with pasteurized bovine milk, and reduced significantly the growth of Bacillus subtilis in milk. We propose these antimicrobial peptides as potential candidates for integration in bioactive milk and food packaging to prevent bacterial growth and extend the shelf life of food.",

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AB - The shelf life of pasteurized bovine milk is limited by microorganism activity as surviving bacteria continue to grow in the bovine milk, eventually causing milk spoilage. In the current study, we used matrix-assisted laser desorption ionization time of flight mass spectrometry to identify pasteurized bovine milk-associated mesophilic and psychrotrophic bacteria. We have recently designed random cationic peptide mixtures that possess strong antimicrobial and antibiofilm properties. These compounds are cheap and easy to synthesize and represent a new class of antimicrobial agents. Here, we show that the random peptide mixtures are able to efficiently eradicate the bacteria identified as associated with pasteurized bovine milk, and reduced significantly the growth of Bacillus subtilis in milk. We propose these antimicrobial peptides as potential candidates for integration in bioactive milk and food packaging to prevent bacterial growth and extend the shelf life of food.

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Random mixtures of antimicrobial peptides inhibit bacteria associated with pasteurized bovine milk

Abstract

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Keywords

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