TY - JOUR
T1 - Rapid diagnosis of old world leishmaniasis by high-resolution melting analysis of the 7sl RNA gene
AU - Nasereddin, Abedelmajeed
AU - Jaffe, Charles L.
PY - 2010/6
Y1 - 2010/6
N2 - High-resolution melt analysis PCR (HRM PCR) for diagnosis of Old World Leishmania was developed using the 7SL RNA gene. Cutaneous leishmaniasis samples were analyzed. Sensitivity and specificity of HRM PCR were significantly better (P < 0.001) than those of internal transcribed spacer 1 PCR and similar to those of kinetoplast DNA PCR.
AB - High-resolution melt analysis PCR (HRM PCR) for diagnosis of Old World Leishmania was developed using the 7SL RNA gene. Cutaneous leishmaniasis samples were analyzed. Sensitivity and specificity of HRM PCR were significantly better (P < 0.001) than those of internal transcribed spacer 1 PCR and similar to those of kinetoplast DNA PCR.
UR - http://www.scopus.com/inward/record.url?scp=77952919811&partnerID=8YFLogxK
U2 - 10.1128/JCM.00553-10
DO - 10.1128/JCM.00553-10
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C2 - 20392923
AN - SCOPUS:77952919811
SN - 0095-1137
VL - 48
SP - 2240
EP - 2242
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 6
ER -