Rapid diagnosis of old world leishmaniasis by high-resolution melting analysis of the 7sl RNA gene

Abedelmajeed Nasereddin, Charles L. Jaffe

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

High-resolution melt analysis PCR (HRM PCR) for diagnosis of Old World Leishmania was developed using the 7SL RNA gene. Cutaneous leishmaniasis samples were analyzed. Sensitivity and specificity of HRM PCR were significantly better (P < 0.001) than those of internal transcribed spacer 1 PCR and similar to those of kinetoplast DNA PCR.

Original languageEnglish
Pages (from-to)2240-2242
Number of pages3
JournalJournal of Clinical Microbiology
Volume48
Issue number6
DOIs
StatePublished - Jun 2010

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