Abstract
High-resolution melt analysis PCR (HRM PCR) for diagnosis of Old World Leishmania was developed using the 7SL RNA gene. Cutaneous leishmaniasis samples were analyzed. Sensitivity and specificity of HRM PCR were significantly better (P < 0.001) than those of internal transcribed spacer 1 PCR and similar to those of kinetoplast DNA PCR.
| Original language | English |
|---|---|
| Pages (from-to) | 2240-2242 |
| Number of pages | 3 |
| Journal | Journal of Clinical Microbiology |
| Volume | 48 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 2010 |
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