Rapid differentiation of Mycobacterium tuberculosis and M. bovis by high-resolution melt curve analysis

Suheir Ereqat; Gila Kahila Bar-Gal; Abedelmajeed Nasereddin; Kifaya Azmi; Sharif E. Qaddomi; Charles L. Greenblatt; Mark Spigelman; Ziad Abdeen

doi:10.1128/JCM.00943-10

Rapid differentiation of Mycobacterium tuberculosis and M. bovis by high-resolution melt curve analysis

Suheir Ereqat^*, Gila Kahila Bar-Gal, Abedelmajeed Nasereddin, Kifaya Azmi, Sharif E. Qaddomi, Charles L. Greenblatt, Mark Spigelman, Ziad Abdeen

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Identification and characterization of the Mycobacterium tuberculosis strains are important for clinical and therapeutic management of tuberculosis. Real-time PCR with a high-resolution melt assay was found to improve the diagnostic process. The assay includes differentiation between M. tuberculosis and Mycobacterium bovis based on one single-nucleotide polymorphism (SNP) in the narGHJI and oxyR genes and determination of M. bovis based on the region of differences 1 (RD1). This assay correctly identified the 7 tested Mycobacterium reference strains and 52 clinical samples with a sensitivity of 2 pg DNA. This assay will help in prescribing adequate treatment and monitoring disease dynamics.

Original language	English
Pages (from-to)	4269-4272
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	48
Issue number	11
DOIs	https://doi.org/10.1128/JCM.00943-10
State	Published - Nov 2010

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abstract = "Identification and characterization of the Mycobacterium tuberculosis strains are important for clinical and therapeutic management of tuberculosis. Real-time PCR with a high-resolution melt assay was found to improve the diagnostic process. The assay includes differentiation between M. tuberculosis and Mycobacterium bovis based on one single-nucleotide polymorphism (SNP) in the narGHJI and oxyR genes and determination of M. bovis based on the region of differences 1 (RD1). This assay correctly identified the 7 tested Mycobacterium reference strains and 52 clinical samples with a sensitivity of 2 pg DNA. This assay will help in prescribing adequate treatment and monitoring disease dynamics.",

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AU - Bar-Gal, Gila Kahila

AU - Nasereddin, Abedelmajeed

AU - Azmi, Kifaya

AU - Qaddomi, Sharif E.

AU - Greenblatt, Charles L.

AU - Spigelman, Mark

AU - Abdeen, Ziad

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AB - Identification and characterization of the Mycobacterium tuberculosis strains are important for clinical and therapeutic management of tuberculosis. Real-time PCR with a high-resolution melt assay was found to improve the diagnostic process. The assay includes differentiation between M. tuberculosis and Mycobacterium bovis based on one single-nucleotide polymorphism (SNP) in the narGHJI and oxyR genes and determination of M. bovis based on the region of differences 1 (RD1). This assay correctly identified the 7 tested Mycobacterium reference strains and 52 clinical samples with a sensitivity of 2 pg DNA. This assay will help in prescribing adequate treatment and monitoring disease dynamics.

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Rapid differentiation of Mycobacterium tuberculosis and M. bovis by high-resolution melt curve analysis

Abstract

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