Rapid differentiation of Mycobacterium tuberculosis and M. bovis by high-resolution melt curve analysis

Suheir Ereqat*, Gila Kahila Bar-Gal, Abedelmajeed Nasereddin, Kifaya Azmi, Sharif E. Qaddomi, Charles L. Greenblatt, Mark Spigelman, Ziad Abdeen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Identification and characterization of the Mycobacterium tuberculosis strains are important for clinical and therapeutic management of tuberculosis. Real-time PCR with a high-resolution melt assay was found to improve the diagnostic process. The assay includes differentiation between M. tuberculosis and Mycobacterium bovis based on one single-nucleotide polymorphism (SNP) in the narGHJI and oxyR genes and determination of M. bovis based on the region of differences 1 (RD1). This assay correctly identified the 7 tested Mycobacterium reference strains and 52 clinical samples with a sensitivity of 2 pg DNA. This assay will help in prescribing adequate treatment and monitoring disease dynamics.

Original languageAmerican English
Pages (from-to)4269-4272
Number of pages4
JournalJournal of Clinical Microbiology
Volume48
Issue number11
DOIs
StatePublished - Nov 2010

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