Rapid DNA fragmentation from hypoxia along the thick ascending limb of rat kidneys

Ronen Beeri, Zvi Symon, Mayer Brezis*, Samuel A. Ben-Sasson, Paul H. Baehr, Seymour Rosen, Richard A. Zager

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

129 Scopus citations

Abstract

Extensive DNA fragmentation, a marker for programmed cell death, was selectively and rapidly induced by hypoxia in the thick ascending limbs of rat kidneys. In isolated perfused kidneys, DNA breaks were present in medullary tubules as early as after 10 minutes of local hypoxia and were prevented by reduction of metabolic work. In a model of radiocontrast-induced acute renal failure, DNA breaks were detected selectively along thick ascending limbs as early as 15 minutes following insult, preceding overt morphological damage. Hypoxia induces rapid DNA fragmentation along thick ascending limbs, where programmed cell death could play an important role in nephron injury and kidney failure.

Original languageAmerican English
Pages (from-to)1806-1810
Number of pages5
JournalKidney International
Volume47
Issue number6
DOIs
StatePublished - Jun 1995

Bibliographical note

Funding Information:
This work was supported by a grant from the US-Israel Binational

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