Rapid liquid chromatography-ultraviolet determination of organic acids and phenolic compounds in red wine and must

Zohar Kerem; Ben Ami Bravdo; Oded Shoseyov; Yizhar Tugendhaft

doi:10.1016/j.chroma.2004.08.105

Rapid liquid chromatography-ultraviolet determination of organic acids and phenolic compounds in red wine and must

Zohar Kerem, Ben Ami Bravdo, Oded Shoseyov, Yizhar Tugendhaft

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

A reversed-phase liquid chromatography-ultraviolet (LC-UV) method is proposed for the rapid simultaneous analysis of the main carboxylic acids and polyphenols in must and wine. Good resolution was obtained for citric, tartaric, malic, lactic, acetic, caffeic, ellagic and gallic acids, (-)-epicatechin, quercetin and resveratrol. A novel silica-based column containing ether-linked phenyl groups, with polar end-capping and suitable for low-pH aqueous mobile phases was used and found to be superior to others tested. The method employed a mixture of 0.2% TFA in water and acetonitrile as eluents, showed linearity and precision, and was applied to samples of must and wine.

Original language	English
Pages (from-to)	211-215
Number of pages	5
Journal	Journal of Chromatography A
Volume	1052
Issue number	1-2
DOIs	https://doi.org/10.1016/j.chroma.2004.08.105
State	Published - 15 Oct 2004

Bibliographical note

Funding Information:
This work was supported in part by a scholarship to Y.T. from the Molcho Center and by the Zigmond and Estel Lovenberg Fund. We wish to thank Mrs. P. Weinberg for her technical support.

Keywords

Carboxylic acids
Must
Organic acids
Polyphenols
Wine

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10.1016/j.chroma.2004.08.105

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title = "Rapid liquid chromatography-ultraviolet determination of organic acids and phenolic compounds in red wine and must",

abstract = "A reversed-phase liquid chromatography-ultraviolet (LC-UV) method is proposed for the rapid simultaneous analysis of the main carboxylic acids and polyphenols in must and wine. Good resolution was obtained for citric, tartaric, malic, lactic, acetic, caffeic, ellagic and gallic acids, (-)-epicatechin, quercetin and resveratrol. A novel silica-based column containing ether-linked phenyl groups, with polar end-capping and suitable for low-pH aqueous mobile phases was used and found to be superior to others tested. The method employed a mixture of 0.2% TFA in water and acetonitrile as eluents, showed linearity and precision, and was applied to samples of must and wine.",

keywords = "Carboxylic acids, Must, Organic acids, Polyphenols, Wine",

author = "Zohar Kerem and Bravdo, {Ben Ami} and Oded Shoseyov and Yizhar Tugendhaft",

note = "Funding Information: This work was supported in part by a scholarship to Y.T. from the Molcho Center and by the Zigmond and Estel Lovenberg Fund. We wish to thank Mrs. P. Weinberg for her technical support.",

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AU - Bravdo, Ben Ami

AU - Shoseyov, Oded

AU - Tugendhaft, Yizhar

N1 - Funding Information: This work was supported in part by a scholarship to Y.T. from the Molcho Center and by the Zigmond and Estel Lovenberg Fund. We wish to thank Mrs. P. Weinberg for her technical support.

PY - 2004/10/15

Y1 - 2004/10/15

N2 - A reversed-phase liquid chromatography-ultraviolet (LC-UV) method is proposed for the rapid simultaneous analysis of the main carboxylic acids and polyphenols in must and wine. Good resolution was obtained for citric, tartaric, malic, lactic, acetic, caffeic, ellagic and gallic acids, (-)-epicatechin, quercetin and resveratrol. A novel silica-based column containing ether-linked phenyl groups, with polar end-capping and suitable for low-pH aqueous mobile phases was used and found to be superior to others tested. The method employed a mixture of 0.2% TFA in water and acetonitrile as eluents, showed linearity and precision, and was applied to samples of must and wine.

AB - A reversed-phase liquid chromatography-ultraviolet (LC-UV) method is proposed for the rapid simultaneous analysis of the main carboxylic acids and polyphenols in must and wine. Good resolution was obtained for citric, tartaric, malic, lactic, acetic, caffeic, ellagic and gallic acids, (-)-epicatechin, quercetin and resveratrol. A novel silica-based column containing ether-linked phenyl groups, with polar end-capping and suitable for low-pH aqueous mobile phases was used and found to be superior to others tested. The method employed a mixture of 0.2% TFA in water and acetonitrile as eluents, showed linearity and precision, and was applied to samples of must and wine.

KW - Carboxylic acids

KW - Must

KW - Organic acids

KW - Polyphenols

KW - Wine

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ER -

Rapid liquid chromatography-ultraviolet determination of organic acids and phenolic compounds in red wine and must

Abstract

Bibliographical note

Keywords

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