Rate of synthesis and concentration of specific mRNA sequences in cultured Chinese hamster ovary cells compared to liver cells

H. Soreq; Michael Harpold; Michael Wilson; J. E. Darnell

doi:10.1016/0006-291X(80)90359-9

Rate of synthesis and concentration of specific mRNA sequences in cultured Chinese hamster ovary cells compared to liver cells

H. Soreq^*, Michael Harpold, Michael Wilson, J. E. Darnell

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

With the aid of recombinant DNA molecules the concentration of nine different mRNAs in cultured Chinese hamster cells has been compared to liver cells. One clone in nine showed a marked increase (13-fold) in liver cells but the remainder were present at about the same level in the two cell types. The rate of RNA synthesis in the nucleus was not increased by 13-fold for this clone but only about 3-fold. These data show that quantitative hybridization to recombinant DNA can estimate mRNA concentrations and rates of synthesis accurately and suggest levels at which control operates.

Original language	English
Pages (from-to)	485-491
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	92
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(80)90359-9
State	Published - 29 Jan 1980
Externally published	Yes

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AU - Darnell, J. E.

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AB - With the aid of recombinant DNA molecules the concentration of nine different mRNAs in cultured Chinese hamster cells has been compared to liver cells. One clone in nine showed a marked increase (13-fold) in liver cells but the remainder were present at about the same level in the two cell types. The rate of RNA synthesis in the nucleus was not increased by 13-fold for this clone but only about 3-fold. These data show that quantitative hybridization to recombinant DNA can estimate mRNA concentrations and rates of synthesis accurately and suggest levels at which control operates.

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Rate of synthesis and concentration of specific mRNA sequences in cultured Chinese hamster ovary cells compared to liver cells

Abstract

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