Readenylation of Polyadenylate‐Free Globin Messenger RNA Restores Its Stability in vivo

Georges HUEZ; Gérard MARBAIX; Evelyne HUBERT; Yvette CLEUTER; Madeleine LECLERCQ; Hubert CHANTRENNE; René DEVOS; Hermona SOREQ; Uri NUDEL; Uriel Z. LITTAUER

doi:10.1111/j.1432-1033.1975.tb02486.x

Readenylation of Polyadenylate‐Free Globin Messenger RNA Restores Its Stability in vivo

Georges HUEZ^*, Gérard MARBAIX, Evelyne HUBERT, Yvette CLEUTER, Madeleine LECLERCQ, Hubert CHANTRENNE, René DEVOS, Hermona SOREQ, Uri NUDEL, Uriel Z. LITTAUER

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

Using an ATP:RNA adenyltransferase from Escherichia coli, a polyadenylic sequence was resynthesized onto rabbit globin mRNA from which the poly(A) segment had been previously removed. Conditions for obtaining a homogenous reconstituted globin mRNA preparation containing 30 adenylic residues per message molecule were determined. The reconstituted globin mRNA was microinjected into Xenopus laevis oocytes. Its stability was very similar to that of native mRNA.

Original language	English
Pages (from-to)	589-592
Number of pages	4
Journal	European Journal of Biochemistry
Volume	59
Issue number	2
DOIs	https://doi.org/10.1111/j.1432-1033.1975.tb02486.x
State	Published - Nov 1975
Externally published	Yes

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10.1111/j.1432-1033.1975.tb02486.x

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title = "Readenylation of Polyadenylate‐Free Globin Messenger RNA Restores Its Stability in vivo",

abstract = "Using an ATP:RNA adenyltransferase from Escherichia coli, a polyadenylic sequence was resynthesized onto rabbit globin mRNA from which the poly(A) segment had been previously removed. Conditions for obtaining a homogenous reconstituted globin mRNA preparation containing 30 adenylic residues per message molecule were determined. The reconstituted globin mRNA was microinjected into Xenopus laevis oocytes. Its stability was very similar to that of native mRNA.",

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AU - HUEZ, Georges

AU - MARBAIX, Gérard

AU - HUBERT, Evelyne

AU - CLEUTER, Yvette

AU - LECLERCQ, Madeleine

AU - CHANTRENNE, Hubert

AU - DEVOS, René

AU - SOREQ, Hermona

AU - NUDEL, Uri

AU - LITTAUER, Uriel Z.

PY - 1975/11

Y1 - 1975/11

N2 - Using an ATP:RNA adenyltransferase from Escherichia coli, a polyadenylic sequence was resynthesized onto rabbit globin mRNA from which the poly(A) segment had been previously removed. Conditions for obtaining a homogenous reconstituted globin mRNA preparation containing 30 adenylic residues per message molecule were determined. The reconstituted globin mRNA was microinjected into Xenopus laevis oocytes. Its stability was very similar to that of native mRNA.

AB - Using an ATP:RNA adenyltransferase from Escherichia coli, a polyadenylic sequence was resynthesized onto rabbit globin mRNA from which the poly(A) segment had been previously removed. Conditions for obtaining a homogenous reconstituted globin mRNA preparation containing 30 adenylic residues per message molecule were determined. The reconstituted globin mRNA was microinjected into Xenopus laevis oocytes. Its stability was very similar to that of native mRNA.

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JO - European Journal of Biochemistry

JF - European Journal of Biochemistry

IS - 2

ER -

Readenylation of Polyadenylate‐Free Globin Messenger RNA Restores Its Stability in vivo

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