Real-time bioluminescence functional imaging for monitoring tissue formation and regeneration

Nadav Bleich Kimelman, Ilan Kallai, Dmitriy Sheyn, Wafa Tawackoli, Zulma Gazit, Gadi Pelled, Dan Gazit

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

4 Scopus citations

Abstract

Real-time bioluminescence functional imaging holds great promise for regenerative medicine because it improves the researcher's ability to analyze and understand the healing process. Using transgenic mice coupled with gene-modified cells, one can employ this method to monitor host and graft activity in various models of tissue regeneration. We implemented real-time bioluminescence functional imaging to analyze bone formation by following a unique protocol in which the luciferase reporter gene, driven by an osteocalcin promoter, is used to visualize host and graft activity during bone formation. Real-time bioluminescence functional imaging can be used to assess the "host reaction" in transgenic mice models; it can also be used to assess "graft activity" in other animals in which genetically labeled stem cells have been implanted or direct gene delivery has been applied. The suggested imaging protocol requires 25 min per sample. However, special attention must be given to the layout of the experimental design, which determines the specific activity that will be analyzed.

Original languageEnglish
Title of host publicationBiological Aging
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages181-193
Number of pages13
ISBN (Print)9781627035552
DOIs
StatePublished - 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1048
ISSN (Print)1064-3745

Keywords

  • Bioluminescence imaging
  • Bone regeneration
  • Imaging analysis
  • Osteocalcin
  • Tissue engineering

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