Recombinant inbred lines for genetic mapping in tomato

I. Paran; I. Goldman; S. D. Tanksley; D. Zamir

doi:10.1007/BF00222001

Recombinant inbred lines for genetic mapping in tomato

I. Paran, I. Goldman, S. D. Tanksley, D. Zamir^*

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

92 Scopus citations

Abstract

A cross between the cultivated tomato Lycopersicon esculentum and a related wild species L. cheesmanii yielded 97 recombinant inbred lines (RILs) which were used to construct a genetic map consisting of 132 molecular markers. Significant deviation from the expected 1:1 ratio between the two homozygous classes was found in 73% of the markers. In 98% of the deviating markers, L. esculentum alleles were present in greater frequency than the L. cheesmanii alleles. For most of the markers with skewed segregation, the direction of the deviation was maintained from F₂ to F₇ generations. The average heterozygosity in the population was 15%. This value is significantly greater than the 1.5% heterozygosity expected for RILs in the F₇ generation. On average, recombination between linked markers was twice as high in the RILs than in the F₂ population used to derive them. The utility of RILs for the mapping of qualitative and quantitative traits is discussed.

Original language	English
Pages (from-to)	542-548
Number of pages	7
Journal	Theoretical And Applied Genetics
Volume	90
Issue number	3-4
DOIs	https://doi.org/10.1007/BF00222001
State	Published - Mar 1995

Keywords

Genetic mapping
Molecular markers
Recombinant inbred lines
Tomato

Access to Document

10.1007/BF00222001

Cite this

@article{578ba973f93d48dba83319f94e7e3dd2,

title = "Recombinant inbred lines for genetic mapping in tomato",

abstract = "A cross between the cultivated tomato Lycopersicon esculentum and a related wild species L. cheesmanii yielded 97 recombinant inbred lines (RILs) which were used to construct a genetic map consisting of 132 molecular markers. Significant deviation from the expected 1:1 ratio between the two homozygous classes was found in 73% of the markers. In 98% of the deviating markers, L. esculentum alleles were present in greater frequency than the L. cheesmanii alleles. For most of the markers with skewed segregation, the direction of the deviation was maintained from F2 to F7 generations. The average heterozygosity in the population was 15%. This value is significantly greater than the 1.5% heterozygosity expected for RILs in the F7 generation. On average, recombination between linked markers was twice as high in the RILs than in the F2 population used to derive them. The utility of RILs for the mapping of qualitative and quantitative traits is discussed.",

keywords = "Genetic mapping, Molecular markers, Recombinant inbred lines, Tomato",

author = "I. Paran and I. Goldman and Tanksley, {S. D.} and D. Zamir",

year = "1995",

month = mar,

doi = "10.1007/BF00222001",

language = "אנגלית",

volume = "90",

pages = "542--548",

journal = "Theoretical And Applied Genetics",

issn = "0040-5752",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "3-4",

}

TY - JOUR

T1 - Recombinant inbred lines for genetic mapping in tomato

AU - Paran, I.

AU - Goldman, I.

AU - Tanksley, S. D.

AU - Zamir, D.

PY - 1995/3

Y1 - 1995/3

N2 - A cross between the cultivated tomato Lycopersicon esculentum and a related wild species L. cheesmanii yielded 97 recombinant inbred lines (RILs) which were used to construct a genetic map consisting of 132 molecular markers. Significant deviation from the expected 1:1 ratio between the two homozygous classes was found in 73% of the markers. In 98% of the deviating markers, L. esculentum alleles were present in greater frequency than the L. cheesmanii alleles. For most of the markers with skewed segregation, the direction of the deviation was maintained from F2 to F7 generations. The average heterozygosity in the population was 15%. This value is significantly greater than the 1.5% heterozygosity expected for RILs in the F7 generation. On average, recombination between linked markers was twice as high in the RILs than in the F2 population used to derive them. The utility of RILs for the mapping of qualitative and quantitative traits is discussed.

AB - A cross between the cultivated tomato Lycopersicon esculentum and a related wild species L. cheesmanii yielded 97 recombinant inbred lines (RILs) which were used to construct a genetic map consisting of 132 molecular markers. Significant deviation from the expected 1:1 ratio between the two homozygous classes was found in 73% of the markers. In 98% of the deviating markers, L. esculentum alleles were present in greater frequency than the L. cheesmanii alleles. For most of the markers with skewed segregation, the direction of the deviation was maintained from F2 to F7 generations. The average heterozygosity in the population was 15%. This value is significantly greater than the 1.5% heterozygosity expected for RILs in the F7 generation. On average, recombination between linked markers was twice as high in the RILs than in the F2 population used to derive them. The utility of RILs for the mapping of qualitative and quantitative traits is discussed.

KW - Genetic mapping

KW - Molecular markers

KW - Recombinant inbred lines

KW - Tomato

UR - http://www.scopus.com/inward/record.url?scp=0029152492&partnerID=8YFLogxK

U2 - 10.1007/BF00222001

DO - 10.1007/BF00222001

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

AN - SCOPUS:0029152492

SN - 0040-5752

VL - 90

SP - 542

EP - 548

JO - Theoretical And Applied Genetics

JF - Theoretical And Applied Genetics

IS - 3-4

ER -

Recombinant inbred lines for genetic mapping in tomato

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this