TY - JOUR
T1 - Recombinant ostreolysin induces brown fat-like phenotype in HIB-1B cells
AU - Oren, Tom
AU - Nimri, Lili
AU - Yehuda-Shnaidman, Einav
AU - Staikin, Katy
AU - Hadar, Yitzhak
AU - Friedler, Assaf
AU - Amartely, Hadar
AU - Slutzki, Michal
AU - Pizio, Antonella Di
AU - Niv, Masha Y.
AU - Peri, Irena
AU - Graeve, Lutz
AU - Schwartz, Betty
N1 - Publisher Copyright:
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2017/9
Y1 - 2017/9
N2 - Scope: Brown adipose tissue (BAT) is the main regulator of thermogenesis by increasing energy expenditure through the uncoupling of oxidative metabolism from ATP synthesis. There is a growing body of evidence for BAT being the key responsible organ in combating obesity and its related disorders. Herein we propose the fungal protein ostreolysin (Oly), which has been previously shown to bind to cholesterol-enriched raft-like membrane domains (lipid rafts) of mammalian cells, as a suitable candidate for interaction with brown preadipocytes. The aim of the present study was therefore to characterize the mechanism by which a recombinant version of ostreolysin (rOly) induces brown adipocyte differentiation. Methods and results: Primary isolated brown preadipocytes or HIB-1B brown preadipocyte cells were treated with rOly and the effects on morphology, lipid accumulation, respiration rate, and associated gene and protein expression were measured. rOly upregulated mRNA and protein levels of factors related to brown adipocyte differentiation, induced lipid droplet formation, and increased cellular respiration rate due to expression of uncoupling protein 1. rOly also upregulated β-tubulin expression, and therefore microtubules might be involved in its mechanism of action. Conclusion: rOly promotes brown adipocyte differentiation, suggesting a new mechanism for rOly's contribution to the battle against obesity.
AB - Scope: Brown adipose tissue (BAT) is the main regulator of thermogenesis by increasing energy expenditure through the uncoupling of oxidative metabolism from ATP synthesis. There is a growing body of evidence for BAT being the key responsible organ in combating obesity and its related disorders. Herein we propose the fungal protein ostreolysin (Oly), which has been previously shown to bind to cholesterol-enriched raft-like membrane domains (lipid rafts) of mammalian cells, as a suitable candidate for interaction with brown preadipocytes. The aim of the present study was therefore to characterize the mechanism by which a recombinant version of ostreolysin (rOly) induces brown adipocyte differentiation. Methods and results: Primary isolated brown preadipocytes or HIB-1B brown preadipocyte cells were treated with rOly and the effects on morphology, lipid accumulation, respiration rate, and associated gene and protein expression were measured. rOly upregulated mRNA and protein levels of factors related to brown adipocyte differentiation, induced lipid droplet formation, and increased cellular respiration rate due to expression of uncoupling protein 1. rOly also upregulated β-tubulin expression, and therefore microtubules might be involved in its mechanism of action. Conclusion: rOly promotes brown adipocyte differentiation, suggesting a new mechanism for rOly's contribution to the battle against obesity.
KW - Brown adipocyte differentiation
KW - Brown adipose tissue
KW - Lipid droplet
KW - Obesity
KW - Ostreolysin
UR - http://www.scopus.com/inward/record.url?scp=85020431994&partnerID=8YFLogxK
U2 - 10.1002/mnfr.201700057
DO - 10.1002/mnfr.201700057
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C2 - 28464422
AN - SCOPUS:85020431994
SN - 1613-4125
VL - 61
JO - Molecular Nutrition and Food Research
JF - Molecular Nutrition and Food Research
IS - 9
M1 - 1700057
ER -