Recombinant ostreolysin (rOly) inhibits the anti-adipogenic Hedgehog (Hh) signaling pathway in 3T3-L1 cells

Erez Israeli; Nastacia Adler Berken; Ofer Gover; Eike Waechtershaeuser; Lutz Graeve; Betty Schwartz

doi:10.1016/j.jff.2019.05.028

Recombinant ostreolysin (rOly) inhibits the anti-adipogenic Hedgehog (Hh) signaling pathway in 3T3-L1 cells

Erez Israeli, Nastacia Adler Berken, Ofer Gover, Eike Waechtershaeuser, Lutz Graeve, Betty Schwartz^*

^*Corresponding author for this work

Institute of Biochemistry, Food Science and Nutrition

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Obesity is a nutrition-associated disorder result of an imbalance between energy intake and energy expenditure. Changing adipocytes differentiation patterns is considered as a strategy to treat obesity-related disorders. Recently, much interest is focused on the role of posttranslational modifications of tubulin on adipocyte differentiation. We recently demonstrated that a recombinant version of the fungal protein Ostreolysin (rOly) drastically affects metabolism of adipose tissue. The aim of the present study is to extend our understanding of the in vitro effects of rOly on different adipocytes. We demonstrate that rOly inhibits the anti-adipogenic Hedgehog (Hh) signaling pathway in 3T3-L1 cells. Additionally, rOly affected the gene expression levels of SQSTM1 and Collagen type 1, which are mediated by AMP-activated protein kinase (AMPK) activity in 3T3-L1 cells. We provide a potential molecular mechanistic approach describing that the effect of rOly on adipocytes is mediated by tubulin acetylation and AMPK phosphorylation.

Original language	American English
Pages (from-to)	185-193
Number of pages	9
Journal	Journal of Functional Foods
Volume	59
DOIs	https://doi.org/10.1016/j.jff.2019.05.028
State	Published - Aug 2019

Bibliographical note

Funding Information:
We thank Prof Arieh Gertler and Dr. Gili Salomon (Hebrew University of Jerusalem) for helping in the designing and synthesizing the rOly mutants. We thank Prof Petra Kluger (University of Reutlingen) for support in culturing adipose-derived stem cells. This study was partly supported by Valin Technologies, Yavneh, Israel. E.I was partly supported by the scholarship of the Baden-Württemberg-Stipendium. E.W was partly supported by the scholarship of the Ministry of Science and Arts of the state of Baden- Württemberg. BS and EI conceived and designed the experiments. EI and EW performed most of the experiments and data analysis. OG provided technical assistance. NS synthesized the rOly mutants. LG conceived some of the experiments. BS contributed reagents, materials, and analytical tools. EI and BS wrote the manuscript and all of the authors made critical revisions. The authors declare that there are not conflict of interests

Funding Information:
We thank Prof Arieh Gertler and Dr. Gili Salomon ( Hebrew University of Jerusalem ) for helping in the designing and synthesizing the rOly mutants. We thank Prof Petra Kluger ( University of Reutlingen ) for support in culturing adipose-derived stem cells. This study was partly supported by Valin Technologies , Yavneh, Israel. E.I was partly supported by the scholarship of the Baden-Württemberg-Stipendium. E.W was partly supported by the scholarship of the Ministry of Science and Arts of the state of Baden- Württemberg.

Publisher Copyright:
© 2019 Elsevier Ltd

Keywords

AMPK
Adipose tissue
Cilia
Hedgehog signaling
Ostreolysin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jff.2019.05.028

Cite this

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title = "Recombinant ostreolysin (rOly) inhibits the anti-adipogenic Hedgehog (Hh) signaling pathway in 3T3-L1 cells",

abstract = "Obesity is a nutrition-associated disorder result of an imbalance between energy intake and energy expenditure. Changing adipocytes differentiation patterns is considered as a strategy to treat obesity-related disorders. Recently, much interest is focused on the role of posttranslational modifications of tubulin on adipocyte differentiation. We recently demonstrated that a recombinant version of the fungal protein Ostreolysin (rOly) drastically affects metabolism of adipose tissue. The aim of the present study is to extend our understanding of the in vitro effects of rOly on different adipocytes. We demonstrate that rOly inhibits the anti-adipogenic Hedgehog (Hh) signaling pathway in 3T3-L1 cells. Additionally, rOly affected the gene expression levels of SQSTM1 and Collagen type 1, which are mediated by AMP-activated protein kinase (AMPK) activity in 3T3-L1 cells. We provide a potential molecular mechanistic approach describing that the effect of rOly on adipocytes is mediated by tubulin acetylation and AMPK phosphorylation.",

keywords = "AMPK, Adipose tissue, Cilia, Hedgehog signaling, Ostreolysin",

author = "Erez Israeli and {Adler Berken}, Nastacia and Ofer Gover and Eike Waechtershaeuser and Lutz Graeve and Betty Schwartz",

note = "Funding Information: We thank Prof Arieh Gertler and Dr. Gili Salomon (Hebrew University of Jerusalem) for helping in the designing and synthesizing the rOly mutants. We thank Prof Petra Kluger (University of Reutlingen) for support in culturing adipose-derived stem cells. This study was partly supported by Valin Technologies, Yavneh, Israel. E.I was partly supported by the scholarship of the Baden-W{\"u}rttemberg-Stipendium. E.W was partly supported by the scholarship of the Ministry of Science and Arts of the state of Baden- W{\"u}rttemberg. BS and EI conceived and designed the experiments. EI and EW performed most of the experiments and data analysis. OG provided technical assistance. NS synthesized the rOly mutants. LG conceived some of the experiments. BS contributed reagents, materials, and analytical tools. EI and BS wrote the manuscript and all of the authors made critical revisions. The authors declare that there are not conflict of interests Funding Information: We thank Prof Arieh Gertler and Dr. Gili Salomon ( Hebrew University of Jerusalem ) for helping in the designing and synthesizing the rOly mutants. We thank Prof Petra Kluger ( University of Reutlingen ) for support in culturing adipose-derived stem cells. This study was partly supported by Valin Technologies , Yavneh, Israel. E.I was partly supported by the scholarship of the Baden-W{\"u}rttemberg-Stipendium. E.W was partly supported by the scholarship of the Ministry of Science and Arts of the state of Baden- W{\"u}rttemberg. Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",

year = "2019",

month = aug,

doi = "10.1016/j.jff.2019.05.028",

language = "American English",

volume = "59",

pages = "185--193",

journal = "Journal of Functional Foods",

issn = "1756-4646",

publisher = "Elsevier Ltd.",

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AU - Israeli, Erez

AU - Adler Berken, Nastacia

AU - Gover, Ofer

AU - Waechtershaeuser, Eike

AU - Graeve, Lutz

AU - Schwartz, Betty

N1 - Funding Information: We thank Prof Arieh Gertler and Dr. Gili Salomon (Hebrew University of Jerusalem) for helping in the designing and synthesizing the rOly mutants. We thank Prof Petra Kluger (University of Reutlingen) for support in culturing adipose-derived stem cells. This study was partly supported by Valin Technologies, Yavneh, Israel. E.I was partly supported by the scholarship of the Baden-Württemberg-Stipendium. E.W was partly supported by the scholarship of the Ministry of Science and Arts of the state of Baden- Württemberg. BS and EI conceived and designed the experiments. EI and EW performed most of the experiments and data analysis. OG provided technical assistance. NS synthesized the rOly mutants. LG conceived some of the experiments. BS contributed reagents, materials, and analytical tools. EI and BS wrote the manuscript and all of the authors made critical revisions. The authors declare that there are not conflict of interests Funding Information: We thank Prof Arieh Gertler and Dr. Gili Salomon ( Hebrew University of Jerusalem ) for helping in the designing and synthesizing the rOly mutants. We thank Prof Petra Kluger ( University of Reutlingen ) for support in culturing adipose-derived stem cells. This study was partly supported by Valin Technologies , Yavneh, Israel. E.I was partly supported by the scholarship of the Baden-Württemberg-Stipendium. E.W was partly supported by the scholarship of the Ministry of Science and Arts of the state of Baden- Württemberg. Publisher Copyright: © 2019 Elsevier Ltd

PY - 2019/8

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N2 - Obesity is a nutrition-associated disorder result of an imbalance between energy intake and energy expenditure. Changing adipocytes differentiation patterns is considered as a strategy to treat obesity-related disorders. Recently, much interest is focused on the role of posttranslational modifications of tubulin on adipocyte differentiation. We recently demonstrated that a recombinant version of the fungal protein Ostreolysin (rOly) drastically affects metabolism of adipose tissue. The aim of the present study is to extend our understanding of the in vitro effects of rOly on different adipocytes. We demonstrate that rOly inhibits the anti-adipogenic Hedgehog (Hh) signaling pathway in 3T3-L1 cells. Additionally, rOly affected the gene expression levels of SQSTM1 and Collagen type 1, which are mediated by AMP-activated protein kinase (AMPK) activity in 3T3-L1 cells. We provide a potential molecular mechanistic approach describing that the effect of rOly on adipocytes is mediated by tubulin acetylation and AMPK phosphorylation.

AB - Obesity is a nutrition-associated disorder result of an imbalance between energy intake and energy expenditure. Changing adipocytes differentiation patterns is considered as a strategy to treat obesity-related disorders. Recently, much interest is focused on the role of posttranslational modifications of tubulin on adipocyte differentiation. We recently demonstrated that a recombinant version of the fungal protein Ostreolysin (rOly) drastically affects metabolism of adipose tissue. The aim of the present study is to extend our understanding of the in vitro effects of rOly on different adipocytes. We demonstrate that rOly inhibits the anti-adipogenic Hedgehog (Hh) signaling pathway in 3T3-L1 cells. Additionally, rOly affected the gene expression levels of SQSTM1 and Collagen type 1, which are mediated by AMP-activated protein kinase (AMPK) activity in 3T3-L1 cells. We provide a potential molecular mechanistic approach describing that the effect of rOly on adipocytes is mediated by tubulin acetylation and AMPK phosphorylation.

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KW - Cilia

KW - Hedgehog signaling

KW - Ostreolysin

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DO - 10.1016/j.jff.2019.05.028

M3 - Article

AN - SCOPUS:85066265645

SN - 1756-4646

VL - 59

SP - 185

EP - 193

JO - Journal of Functional Foods

JF - Journal of Functional Foods

ER -

Recombinant ostreolysin (rOly) inhibits the anti-adipogenic Hedgehog (Hh) signaling pathway in 3T3-L1 cells

Abstract

Bibliographical note

Keywords

UN SDGs

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