Reduced expression of the v-SNAREs AtVAMP71/AtVAMP7C gene family in Arabidopsis reduces drought tolerance by suppression of abscisic acid-dependent stomatal closure

Yehoram Leshem, Yael Golani, Yuval Kaye, Alex Levine*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

Stomatal closure during water stress is a major plant mechanism for reducing the loss of water through leaves. The opening and closure of stomata are mediated by endomembrane trafficking. The role of the vacuolar trafficking pathway, that involves v-SNAREs of the AtVAMP71 family (formerly called AtVAMP7C) in stomatal movements, was analysed. Expression of AtVAMP711-14 genes was manipulated in Arabidopsis plants with sense or antisense constructs by transformation of the AtVAMP711 gene. Antisense plants exhibited decreased stomatal closure during drought or after treatment with abscisic acid (ABA), resulting in the rapid loss of leaf water and tissue collapse. No improvement was seen in plants overexpressing the AtVAMP711 gene, suggesting that wild-type levels of AtVAMP711 expression are sufficient. ABA treatment induced the production of reactive oxygen species (ROS) in guard cells of both wild-type and antisense plants, indicating that correct hormone sensing is maintained. ROS were detected in nuclei, chloroplasts, and vacuoles. ABA treatment caused a significant increase in ROS-containing small vacuoles and also in plastids and nuclei of neighbouring epidermal and mesophyll cells. Taken together, our results show that VAMP71 proteins play an important role in the localization of ROS, and in the regulation of stomatal closure by ABA treatment. The paper also describes a novel aspect of ROS signalling in plants: that of ROS production in small vacuoles that are dispersed in the cytoplasm.

Original languageAmerican English
Pages (from-to)2615-2622
Number of pages8
JournalJournal of Experimental Botany
Volume61
Issue number10
DOIs
StatePublished - 2010

Bibliographical note

Funding Information:
We thank Dr Naomi Melamed-Book for assistance with confocal microcopy and J Manor and S Arkin for preparation of liposomes. We thank the Canadian Friends of the Hebrew University of Jerusalem for financial aid to YG and YK. The research was supported by a grant from the Israel Science Foundation. Author Disclosure Statement: No competing financial interests exist.

Keywords

  • Abiotic stress
  • Oxidative stress
  • Phosphatidylinositol
  • SNAREs
  • Vesicle

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