TY - JOUR
T1 - Reduction of tellurite and deesterification of fluorescein diacetate are not well correlated with the viability of mycobacteria
AU - Mor, N.
AU - Resnick, M.
AU - Silbaq, F.
AU - Bercovier, H.
AU - Levy, L.
PY - 1988
Y1 - 1988
N2 - Both Mycobacterium leprae and M. lepraemurium (MLM) were capable of reducing tellurium as tellurite ion (Te4+) to elemental tellurium (Te), seen by electron microscopy as fine crystals within the bacterial cells. There appeared to be close correspondence between the capacity to reduce tellurite, bright green fluorescence after staining with fluorescein diacetate (FDA) and the ability of M. smegmatis to multiply in culture. Likewise, there appeared to be correspondence between tellurite reduction and fluorescence after FDA staining for MLM subjected to prolonged storage in the cold or to heating at 70°C. However, correspondence with tellurite-reduction or fluorescence after FDA staining was not observed when death of MLM occured.
AB - Both Mycobacterium leprae and M. lepraemurium (MLM) were capable of reducing tellurium as tellurite ion (Te4+) to elemental tellurium (Te), seen by electron microscopy as fine crystals within the bacterial cells. There appeared to be close correspondence between the capacity to reduce tellurite, bright green fluorescence after staining with fluorescein diacetate (FDA) and the ability of M. smegmatis to multiply in culture. Likewise, there appeared to be correspondence between tellurite reduction and fluorescence after FDA staining for MLM subjected to prolonged storage in the cold or to heating at 70°C. However, correspondence with tellurite-reduction or fluorescence after FDA staining was not observed when death of MLM occured.
KW - Fluorescein
KW - Mycobacterium leprae
KW - Mycobacterium lepraemurium
KW - Mycobacterium smegmatis
KW - Tellurite
KW - Viability
UR - http://www.scopus.com/inward/record.url?scp=0023886683&partnerID=8YFLogxK
U2 - 10.1016/0769-2609(88)90019-1
DO - 10.1016/0769-2609(88)90019-1
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C2 - 3179056
AN - SCOPUS:0023886683
SN - 0769-2609
VL - 139
SP - 279
EP - 288
JO - Annales de l'Institut Pasteur Microbiology
JF - Annales de l'Institut Pasteur Microbiology
IS - 3
ER -