Regulation of acid phosphatase in reverse micellar system by lipids additives: Structural aspects

E. V. Kudryashova*, V. L. Bronza, A. A. Vinogradov, A. Kamyshny, S. Magdassi, A. V. Levashov

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Reverse micelles system is suggested as a direct tool to study the influence of membrane matrix composition on the activity and structure of membrane-associated enzymes with the use of acid phosphatase (AP) as an example. In reverse micelles the functioning of the monomeric and dimeric forms of AP could be separately observed by variation of the size of the micelles. We found that including the lipids into the micellar system can dramatically affect the enzyme functioning even at low lipid content (2% w/w), and this effect depends on the lipid nature. Structural studies using CD spectroscopy and DLS methods have shown that the influence of lipid composition on the enzyme properties might be caused by the interaction of lipids with the enzyme as well as by the influence of lipids on structure and properties of the micellar matrix.

Original languageAmerican English
Pages (from-to)490-497
Number of pages8
JournalJournal of Colloid and Interface Science
Volume353
Issue number2
DOIs
StatePublished - 15 Jan 2011

Bibliographical note

Funding Information:
This work was partially supported by Israeli Government.

Keywords

  • Acid phosphatase
  • Circular dichroism
  • DLS
  • Membrane lipids
  • Reverse micelles

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