Regulation of chemokines and chemokine receptors after experimental closed head injury

Viviane I. Otto; Philip F. Stahel; Mario Rancan; Karin Kariya; Esther Shohami; Ido Yatsiv; Hans Pietro Eugster; Thomas Kossmann; Otmar Trentz; Maria C. Morganti-Kossmann

doi:10.1097/00001756-200107030-00053

Regulation of chemokines and chemokine receptors after experimental closed head injury

Viviane I. Otto, Philip F. Stahel^*, Mario Rancan, Karin Kariya, Esther Shohami, Ido Yatsiv, Hans Pietro Eugster, Thomas Kossmann, Otmar Trentz, Maria C. Morganti-Kossmann

^*Corresponding author for this work

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

The expression of the chemokines macrophage inflammatory protein (MIP)-2 and MIP-Iα and of their receptors CXCR2 and CCR5 was assessed in wild type (WT) and TNF/lymphotoxin-α knockout (TNF/LT-α-/-) mice subjected to closed head injury (CHI). At 4h after trauma intracerebral MIP-2 and MIP-Iα levels were increased in both groups with MIP-2 concentrations being significantly higher in WT than in TNF/LT-α-/animals (p<0.05). Thereafter, MIP-2 production declined rapidly, whereas MIP-lα remained elevated for 7 days. Expression of CXCR2 was confined to astrocytes and increased dramatically within 24h in both mouse types. Contrarily, CCR5 expression remained constitutively low and was mainly localized to microglia. These results show that after CHI, chemokines and their receptors are regulated differentially and with independent kinetics.

Original language	English
Pages (from-to)	2059-2064
Number of pages	6
Journal	NeuroReport
Volume	12
Issue number	9
DOIs	https://doi.org/10.1097/00001756-200107030-00053
State	Published - 3 Jul 2001

Keywords

Chemokine receptors
Chemokines
Cytokines
Knockout mice
Neuroinflammation
Traumatic brain injury

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10.1097/00001756-200107030-00053

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title = "Regulation of chemokines and chemokine receptors after experimental closed head injury",

abstract = "The expression of the chemokines macrophage inflammatory protein (MIP)-2 and MIP-Iα and of their receptors CXCR2 and CCR5 was assessed in wild type (WT) and TNF/lymphotoxin-α knockout (TNF/LT-α-/-) mice subjected to closed head injury (CHI). At 4h after trauma intracerebral MIP-2 and MIP-Iα levels were increased in both groups with MIP-2 concentrations being significantly higher in WT than in TNF/LT-α-/animals (p<0.05). Thereafter, MIP-2 production declined rapidly, whereas MIP-lα remained elevated for 7 days. Expression of CXCR2 was confined to astrocytes and increased dramatically within 24h in both mouse types. Contrarily, CCR5 expression remained constitutively low and was mainly localized to microglia. These results show that after CHI, chemokines and their receptors are regulated differentially and with independent kinetics.",

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AU - Otto, Viviane I.

AU - Stahel, Philip F.

AU - Rancan, Mario

AU - Kariya, Karin

AU - Shohami, Esther

AU - Yatsiv, Ido

AU - Eugster, Hans Pietro

AU - Kossmann, Thomas

AU - Trentz, Otmar

AU - Morganti-Kossmann, Maria C.

PY - 2001/7/3

Y1 - 2001/7/3

N2 - The expression of the chemokines macrophage inflammatory protein (MIP)-2 and MIP-Iα and of their receptors CXCR2 and CCR5 was assessed in wild type (WT) and TNF/lymphotoxin-α knockout (TNF/LT-α-/-) mice subjected to closed head injury (CHI). At 4h after trauma intracerebral MIP-2 and MIP-Iα levels were increased in both groups with MIP-2 concentrations being significantly higher in WT than in TNF/LT-α-/animals (p<0.05). Thereafter, MIP-2 production declined rapidly, whereas MIP-lα remained elevated for 7 days. Expression of CXCR2 was confined to astrocytes and increased dramatically within 24h in both mouse types. Contrarily, CCR5 expression remained constitutively low and was mainly localized to microglia. These results show that after CHI, chemokines and their receptors are regulated differentially and with independent kinetics.

AB - The expression of the chemokines macrophage inflammatory protein (MIP)-2 and MIP-Iα and of their receptors CXCR2 and CCR5 was assessed in wild type (WT) and TNF/lymphotoxin-α knockout (TNF/LT-α-/-) mice subjected to closed head injury (CHI). At 4h after trauma intracerebral MIP-2 and MIP-Iα levels were increased in both groups with MIP-2 concentrations being significantly higher in WT than in TNF/LT-α-/animals (p<0.05). Thereafter, MIP-2 production declined rapidly, whereas MIP-lα remained elevated for 7 days. Expression of CXCR2 was confined to astrocytes and increased dramatically within 24h in both mouse types. Contrarily, CCR5 expression remained constitutively low and was mainly localized to microglia. These results show that after CHI, chemokines and their receptors are regulated differentially and with independent kinetics.

KW - Chemokine receptors

KW - Chemokines

KW - Cytokines

KW - Knockout mice

KW - Neuroinflammation

KW - Traumatic brain injury

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Regulation of chemokines and chemokine receptors after experimental closed head injury

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Keywords

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