Regulation of fructosyltransferase activity by carbohydrates, in solution and immobilized on hydroxyapatite surfaces

Doron Steinberg*, Ramona Rozen, Moshe Bromshteym, Batia Zaks, Itzhak Gedalia, Gilad Bachrach

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

We tested the effect of several carbohydrates on the activity of cell-free fructosyltransferases (FTF) in solution and immobilized onto hydroxyapatite (HA) and found an inhibitory dose-dependent effect of glucose on FTF activity, both on the surface and in solution. Glucose at 160 mM inhibits FTF activity by 75% both on HA and in solution. Fructose at 160 mM inhibited FTF activity by 25% in solution and by 15% on HA. Levan inhibited FTF activity by 30% in solution, while dextrans and inulin had a limited effect on FTF activity. Circular dichroism and infrared analysis demonstrated no major changes in the chemical structure of fructans synthesized by cell-free FTF on HA and in solution, in the presence or absence of glucose. However, as verified by size-exclusion chromatography, glucose inhibited the synthesis of high molecular-weight fructans. The results indicate that glucose, a byproduct of the FTF enzymatic reaction, is the main carbohydrate affecting FTF activity. Selective inhibition of high molecular-weight fructan production by glucose, may indicate that two mechanisms are involved in the synthesis of fructans, both in solution and on the surface.

Original languageAmerican English
Pages (from-to)701-710
Number of pages10
JournalCarbohydrate Research
Volume337
Issue number8
DOIs
StatePublished - 17 Apr 2002
Externally publishedYes

Keywords

  • Biofilm
  • Dental plaque
  • Fructans
  • Fructosyltransferase
  • Hydroxyapatite

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